The protocadherin alpha cluster is required for axon extension and myelination in the developing central nervous system

作     者：Wen-cheng Lu Yu-xiao Zhou Ping Qiao Jin Zheng Qiang Wu Qiang Shen 

作者机构：Department of Orthopedics Shanghai General Hospital Shanghai Jiao Tong University School of Medicine Center for Comparative Biomedicine Key Laboratory of Systems Biomedicine (Ministry of Education) Institute of Systems Biomedicine Shanghai Center for Systems Biomedicine Shanghai Jiao Tong University Department of Orthopedics People’s Hospital of Zhangqiu 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2018年第13卷第3期

页      面：427-433页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学] 

基　　金：supported by a grant from the Science and Technology Commission of Shanghai Municipality of China No.12441900702 

主　　题：nerve regeneration spinal cord injury axons protocadherin α cluster hippocampal neurons RNA sequencing real-time quantitative polymerase chain reaction transmission electron microscopy neural regeneration 

摘      要：In adult mammals, axon regeneration after central nervous system injury is very poor, resulting in persistent functional loss. Enhancing the ability of axonal outgrowth may be a potential treatment strategy because mature neurons of the adult central nervous system may retain the intrinsic ability to regrow axons after injury. The protocadherin （Pcdh） clusters are thought to function in neuronal morphogenesis and in the assembly of neural circuitry in the brain. We cultured primary hippocampal neurons from E17.5 Pcdhα deletion （del-α） mouse embryos. After culture for 1 day, axon length was obviously shorter in del-α neurons compared with wild-type neurons. RNA sequencing of hippocampal E17.5 RNA showed that expression levels of BDNF, Fmod, Nrp2, OGN, and Sema3d, which are associated with axon extension, were significantly down-regulated in the absence of the Pcdhα gene cluster. Using transmission electron microscopy, the ratio of myelinated nerve fibers in the axons of del-α hippocampal neurons was significantly decreased; myelin sheaths of P21 Pcdhα-del mice showed lamellar disorder, discrete appearance, and vacuoles. These results indicate that the Pcdhα cluster can promote the growth and myelination of axons in the neurodevelopmental stage.