Comparative Research on Facultative Anaerobic Cellulose Decomposing Bacteria Screened from Soil and Rumen Content and Diet of Dairy Cow

作     者：Li Yan-fang Wang Lei Liang Zi-chao Zhang Mei-mei Wang Jing-jing Liu Da-sen 

作者机构：College of Animal Sciences and Technology Northeast Agricultural University College of Science Northeast Agricultural University 

出 版 物：《Journal of Northeast Agricultural University(English Edition)》 (东北农业大学学报（英文版）)

年 卷 期：2018年第25卷第1期

页      面：40-46页

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 

基　　金：Supported by Northeast Agricultural University Innovation Funding for Postgraduate(yjscx14007) China Agriculture Research System(CARS-37) 

主　　题：dairy cow facultative anaerobic cellulose-decomposing bacterium cellulase characteristic 16Sr DNA gene analysis 

摘      要：Twenty-nine facultative anaerobic cellulose-degrading bacteria were isolated from soil, rumen fluid, rumen residues and diet of dairy cow. Based on 16 Sr DNA analysis by BLAST algorithm method, the results showed that most of the strains were Bacillus genera, and six of the 29 strains were bigger than 10 mm of diameter of clear zones. For them, two strains were isolated from rumen fluid(L5 and L7) and other two were isolated from rumen residue(N5 and N9), while others were isolated from soil(T1) and diet(S6), respectively. Strains from rumen fluid and residue had higher activities of FPCase and CMCase, but lower β-glucosidases.