Quinacrine pretreatment reduces microwave-induced neuronal damage by stabilizing the cell membrane

作     者：Xue-feng Ding Yan Wu Wen-rui Qu Ming Fan Yong-qi Zhao 

作者机构：Department of Cognitive Sciences Beijing Institute of Basic Medical Sciences Hand & Foot Surgery and Reparative & Reconstructive Surgery Center Orthopedic Hospital of the Second Hospital of Jilin University 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2018年第13卷第3期

页      面：449-455页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the Integrated Drug Discovery Technology Platform of National Science and Technology Major Projects for "Major New Drugs Innovation and Development",No.2012ZX09J12201-005 the National Natural Science Foundation of China,No.31071042,31200822 a grant of Beijing Natural Science Foundation,No.5122033 

主　　题：nerve regeneration quinacrine microwave irradiation heat shock cell apoptosis cell necrosis thermal effect cell membrane heat shock protein 70 nerve cells neural regeneration 

摘      要：Quinacrine, widely used to treat parasitic diseases, binds to cell membranes. We previously found that quinacrine pretreatment reduced microwave radiation damage in rat hippocampal neurons, but the molecular mechanism remains poorly understood. Considering the thermal effects of microwave radiation and the protective effects of quinacrine on heat damage in cells, we hypothesized that quinacrine would prevent microwave radiation damage to cells in a mechanism associated with cell membrane stability. To test this, we used retinoic acid to induce PC12 cells to differentiate into neuron-like cells. We then pretreated the neurons with quinacrine （20 and 40 mM） and irradiated them with 50 mW/cm^2 microwaves for 3 or 6 hours. Flow cytometry, atomic force microscopy and western blot assays revealed that irradiated cells pretreated with quinacrine showed markedly less apoptosis, necrosis, and membrane damage, and greater expression of heat shock protein 70, than cells exposed to microwave irradiation alone. These results suggest that quinacrine stabilizes the neuronal membrane structure by upregulating the expression of heat shock protein 70, thus reducing neuronal injury caused by microwave radiation.