Effect of Xiaochuanping powder on the inflammatory response and airway remodeling in asthmatic rats

作     者：Zhou Tao Zhang Nianzhi Hu Die Wang Wendong Xu Shunfu Chen Xu 

作者机构：Department of biochemistry and molecular biology Anhui University of Chinese Medicine Department of respiratory medicine the First Affiliated Hospital of Anhui University of Chinese Medicine 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2018年第38卷第1期

页      面：61-66页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by Natural Science Foundation of China:Intervention Mechanism of the Method of Tonifying Qi And Promoting Blood Circulation and Resolving Phlem on Airway Remodeling after COPD(No.81473675) Based on p38MAP Signal Pathway Yiqi Huoxue Huatan Method was Explored the Mechanism Study of Bronchial Asthma Airway Remodeling(No.81774301) Excellent Young Talent Fund Project of Colleges and Universities in Anhui Province:Study on the Mechanism of Airway Remodeling of Asthma Model In Rats With Xiaochuanping Powder(No.2011SQRL093) 

主　　题：Asthma Airway remodeling Eosino- phils Eosinophil cationic protein Matrix metallo- proteinase 9 Tumor necrosis factor-alpha Tissue in- hibitor of metalloproteinase-1 Xiaochuanping powder 

摘      要：OBJECTIVE： To observe the effect of Xiaochuanping powder （XP）, a traditional Chinese prescrip- tion for the treatment of cough and asthma, on serum concentrations of eosinophil cationic protein （ECP）, tumor necrosis factor （TNF）-a, and interleu- kin （IL）-4, eosinophil counts, as well as expression of matrix metalloproteinase （MMP）-9, tissue inhibi- tor of metalloproteinase （TIMP）-I in the lung tissues of asthmatic rats. METHODS： Sixty clean-grade Sprague-Dawley rats were divided randomly into six groups： normal control （NC）, asthma model, Guilong Kechuanning （GK） group, as well as high, intermediate-, and lowdose XP groups. Rats were sensitized with ovalbu- min （OVA） to trigger asthma. Serum concentrations of ECP, TNF-a and IL-4, eosinophil counts, as well as expression of MMP-9 and TIMP-1 in lung tissues were evaluated using an immunofluorescence method, mRNA expression of MMP-9 and TIMP-1 was determined using real-time quantitative poly- merase chain reaction. RESULTS： Compared with the asthma-model group, serum concentrations of ECP, TNF-a, and IL-4, and eosinophil counts decreased significantly in the high- and intermediate-dose XP groups and GK group （all P 〈 0.01）. Protein expression of MMP-9 and TIMP-1 decreased significantly in the high- and intermediate-dose XP groups and GK group （all P 〈 0.01）. Transcription of MMP-9 and TIMP-1 mRNA and the ratio of expression of MMP-9：TIMP-1 in lung tissue were significantly lower （P 〈 0.01）. CONCLUSION： XP can reduce TNF-a secretion, sup- press the infiltration/activation of eosinophils, re- duce serum concentrations of ECP and IL-4, reduce the protein and mRNA expression of MMP-9 and TIMP-1 in lung tissue, and regulate the balance between expression of MMP-9 and TIMP-1. In these ways, XP alleviated the inflammation and remodel- ing of the airways.