Impacts of Active Ingredients of Three Traditional Chinese Medicine on Immune Effect of PCV2 Vaccine
Impacts of Active Ingredients of Three Traditional Chinese Medicine on Immune Effect of PCV2 Vaccine作者机构：Service Center of Agriculture InformationWuxi Council of Agriculture Jiangsu Agri-animal Husbandry Vocational College Animal Diseases Control & Prevention Centre of Wuxi Animal Diseases Control & Prevention Centre of Yixing
出 版 物：《Animal Husbandry and Feed Science》 (动物与饲料科学（英文版）)
年 卷 期：2017年第9卷第6期
页 面：402-406页
学科分类：090603[农学-临床兽医学] 08[工学] 09[农学] 0906[农学-兽医学] 080402[工学-测试计量技术及仪器] 0804[工学-仪器科学与技术]
基 金：Supported by the 333 Project of Jiangsu Province(BRA2016102) Natural Science Foundation of Tibet(ZJ2013018) Phoenix Talent Engineering of Jiangsu Agri-animal Husbandry Vocational College
主 题：Active ingredients of traditional Chinese medicine PCV2 vaccine Humoral immunity Cellular immunity
摘 要：[ Objective ] The paper was to screen the active ingredients of traditional Chinese medicine （ TCM）, which significantly enhanced the immune effect of porcine circovirus type 2 （PCV2） vaccine. [Method]Sixty-six 14-day-old piglets were randomly divided into 11 groups, including immune control group, blank control group and nine drug groups. Piglets that were reared for 21 d were intramuscularly injected with 2 mL of inactivated vaccine in cervical region expect for blank control group. Piglets in nine drug groups were administrated with 5 mL of matrine, scutellarin and astragalus polysaecharide （ASP） at three doses （ high dose 12 mg, medium dose 8 nag, low dose 4 mg） three days before and after immunization; piglets in blank control group and irmnune control group were administrated with equal volume of normal saline. Five piglets were randomly selected from each group to collect venous blood at 7, 14, 21 and 28 d post immunization, and the PCV2 antibody level, the concentration of specific immunoglobulin [gG, the changes in peripheral blood lymphocyte subsets, and the content of serum IL-2 and IFN-γ were determined. [ Result] The immunological effects of active ingredients of three TCM on PCV2 vaccine all enhanced at different degree. The effects of scutellarin middle dose group was the best. [ Conclusion ] Scutellarin could be used as a candidate drug for PCV2 vaccine immunoenhancer.
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