Basic and Clinical Evidence of an Alternative Method to Produce Vivo Nanofat
Basic and Clinical Evidence of an Alternative Method to Produce Vivo Nanofat作者机构：Department of Plastic Surgery Peking University Third Hospital Beijing 100191 China Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology National Engineering Laboratory for Digital and Material Technology of Stomatology Beijing Key Laboratory of Digital Stomatology Beijing 100081 China
出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)
年 卷 期：2018年第131卷第5期
页 面：588-593页
核心收录：
学科分类：0710[理学-生物学] 1002[医学-临床医学] 07[理学] 08[工学] 081201[工学-计算机系统结构] 071002[理学-动物学] 0812[工学-计算机科学与技术（可授工学、理学学位）]
主 题：Adipose Tissue Cell Therapy Mesenchymal Stromal Cells Rejuvenation
摘 要：Background： Fat grafting technologies are popularly used in plastic and reconstructive surgery. Due to its size limitation, it is hard to directly inject untreated iht tissue into the dermal layer. Nanolht, which was introduced by Tonnard, solves this problem by mechanically emulsifying fat tissue. However, the viability of the cells was greatly destroyed. In this study, we reported a new method by ＂gently＂ digesting the fat tissue to produce viable adipocytes, progenitors, and stromal stem cells using collagenase I digestion and centrifugation. This was named ＂Vivo nanofat＂. Methods： Human liposuction aspirates were obtained from five healthy female donors with mean age of 28.7±5.6 years. Colony-forming assay, flow cytometry analysis, and adipogenic and osteogenic induction of the adherent cells from the Vivo nanofat were used to characterize the adipose mesenchymal stem cells （MSCs）. To investigate in vivo survival, we respectively injected Vivo nanofat and nanofat subcutaneously to the back of 8-week-old male BALB/c nude mice. Samples were harvested 2 days, 2 weeks, and 4 weeks postiniection for measurement, hematoxylin and eosin staining, and immunostaining. Results： Our results showed that the Vivo nanofat contained a large number ofcolony-fbrming cells. These cells expressed MSC markers and had multi-differentiative potential. In vivo transplantation showed that the Vivo nanofat had lower resorption ratio than that of nanofat. The size of the transplanted nanofat was obviously smaller than that of Vivo nanofat 4 weeks postinjection （0.50±0.17 cm vs. 0.81 ± 0.07 cm, t = -5783, P- 0.01）. Conclusion： Vivo nanofat may serve as a cell fraction injectable through a fine needle; this could be used for cosmetic applications.
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