Comparative analysis of four active compounds of Baikal skullcap and its classical TCM prescriptions according to different clinical curative effects

作     者：Guang-Wei Zhu Sheng-Jun Ma Ao-Xue Liu 

作者机构：Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences School of Food Sciences and Pharmacy Xinjiang Agriculture University School of Chinese Materia Beijing University of Chinese Medicine 

出 版 物：《World Journal of Traditional Chinese Medicine》 (世界中医药杂志（英文）)

年 卷 期：2017年第3卷第1期

页      面：22-30页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by"the Fundamental Research Funds for the Central public welfare research institutes"(NO:ZXKT15037) "comprehensive large platform of Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences"(NO:Z131 100006513017) 

主　　题：Baikal skullcap Couplet medicine Clinical curative effect Compatibility of medicines Proportions of the chemical compounds 

摘      要：Objective: A sensitive HPLC-DAD detection method was established for the comparative analysis of the four active compounds(including baicalin, baicalein, wogonoside and wogonin) of Baikal Skullcap and its classical TCM prescriptions according to different clinical curative effects. And analyze the relationship between compatibility of medicines, content and clinical curative ***: Water extracts were analyzed by high-performance liquid chromatography-diode array detection(HPLC-DAD), using an Agilent extend-C_(18)(4.6 mm×250 mm,5μm) column by using solvent A(0.2% phosphoric acid water) and solvent B(methanol) in the ratio of 52:48(v/v) at a flow rate of 1 ml/min. The column temperature was maintained at 25°C and the detection wavelength was set at 280 ***: In quantitative analysis, the four selected markers showed good regression(R^20.999) within test ranges. The average recoveries were between 99.75~100.60% and their RSD values were between 0.97%~1.71%. The proportions of the four chemical compounds for different efficacy were 33.51~35.76: 6.78~6.87: 3.45~3.64: 1; 18.6~23.64: 3.63~4.27: 1.36~1.53: 1; 29.94~31.78: 5.14~5.23: 3.28~3.56: 1; 33.99~41.91:5.83~7.42: 2.83~3.39: ***: Clinical practice of thousands of years have proved that the curative effect of Traditional Chinese Medicine depends on the compatibility of different kinds of medicine material crude slices. According to this study we found that different compatibility of medicines leads to different proportions of the chemical compounds. We assume that there is a certain correlation between therapeutical effect of TCM and compatibility of medicines.