TRPC3 is required for the survival, pluripotency and neural differentiation of mouse embryonic stem cells(mESCs)

作     者：Helen Baixia Hao Sarah E. Webb Jianbo Yue Marc Moreau Catherine Leclerc Andrew L. Miller 

作者机构：Division of Life Science and State Key Laboratory of Molecular Neuroscience HKUST Clear Water Bay Hong Kong China Department of Biomedical Sciences City University of Hong Kong Hong Kong China Centre de Biologie du Développement (CBD) Centre de Biologie Intégrative (CBI) Université de Toulouse CNRS UPS Toulouse F-31062 France 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2018年第61卷第3期

页      面：253-265页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：supported by the Hong Kong Research Grants Council(RGC)General Research Fund awards(662113,16101714,16100115) the ANR/RGC joint research scheme award(AHKUST601/13) the Hong Kong Theme-based Research Scheme award(T13-706/11-1) the Hong Kong Innovation and Technology Commission(ITCPD/17-9) 

主　　题：transient receptor potential canonical subfamily member 3 (TRPC3) mouse embryonic stem cells (mESCs) neurondifferentiation CRISPR/Cas9 pluripotency apoptosis mitochondrial membrane potential 

摘      要：Transient receptor potential canonical subfamily member 3(TRPC3) is known to be important for neural development and the formation of neuronal networks. Here, we investigated the role of TRPC3 in undifferentiated mouse embryonic stem cells(mESCs) and during the differentiation of mESCs into neurons. CRISPR/Cas9-mediated knockout(KO) of TRPC3 induced apoptosis and the disruption of mitochondrial membrane potential both in undifferentiated mESCs and in those undergoing neural differentiation. In addition, TRPC3 KO impaired the pluripotency of mESCs. TRPC3 KO also dramatically repressed the neural differentiation of mESCs by inhibiting the expression of markers for neural progenitors, neurons, astrocytes and *** together, our new data demonstrate an important function of TRPC3 with regards to the survival, pluripotency and neural differentiation of mESCs.