Human cytomegalovirus miR-US5-1 inhibits viral replication by targeting Geminin mRNA

作     者：Shujuan Jiang Yujing Huang Ying Qi Rong He Zhongyang Liu Yanping Ma Xin Guo Yaozhong Shao Zhengrong Sun Qiang Ruan 

作者机构：Virus Laboratory the Affiliated Shengjing Hospital China Medical University Shenyang 110122 China Clinical Genetics the Affiliated Shengjing Hospital China Medical University Shenyang 110122 China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2017年第32卷第5期

页      面：431-439页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China (81371788 and 81171580) the Specialized Research Fund for the Doctoral Program of Higher Education (20112104110012) the Outstanding Scientific Fund of Shengjing Hospital 

主　　题：Human cytomegalovirus(HCMV) hcmv-mi R-US5-1 Geminin(GMNN) DNA replication cell cycle 

摘      要：Viruses commonly create favorable cellular conditions for their survival through multiple mechanisms. Micro RNAs(mi RNAs), which function as post-transcriptional regulators, are utilized by human cytomegalovirus(HCMV) in its infection and pathogenesis. In the present study, the DNA replication inhibitor Geminin(GMNN) was identified to be a direct target of hcmv-mi R-US5-1. Overexpression of hcmv-mi R-US5-1 could block the accumulation of GMNN during HCMV infection, and the decrease of GMNN expression caused by hcmv-mi R-US5-1 or GMNN specific si RNA reduced HCMV DNA copies in U373 cells. Meanwhile, ectopic expression of hcmv-mi R-US5-1 and consequent lower expression of GMNN influenced host cell cycle and proliferation. These results imply that hcmv-mi R-US5-1 may affect viral replication and host cellular environment by regulating expression kinetics of GMNN during HCMV infection.