Tissue Culture Rapid Propagation and Transplantation Techniques of Anoectochilus roxburghii (Wall) Lind.

作     者：Xiulian LIN Xinxiao JIANG Zixuan YANG Xiaoyong MA Xuchao YAN Lihua YANG 

作者机构：Huizhou Engineering Vocational College Huizhou Institute of Agricultural Sciences 

出 版 物：《Medicinal Plant》 (药用植物：英文版)

年 卷 期：2018年第9卷第1期

页      面：43-46页

学科分类：09[农学] 0904[农学-植物保护] 

基　　金：Supported by Science and Technology Project of Huizhou City(2014C040007001) 

主　　题：Anoectochilus roxburghii (Wall) Lind Tissue culture rapid propagation Transplantation technique 

摘      要：[Objectives]To screen the tissue culture rapid propagation formula suitable for each tissue culture stage of Anoectochilus roxburghii( Wall) Lind. [Methods] The stem segments of Fujian A. roxburghii were used as explant to study the tissue culture rapid propagation and transplantation techniques. The comparative experiment was carried out to study the effects of different hormone concentrations on the induction of stem segments,proliferation of cluster buds,rooting and seedling hardening of A. roxburghii,and study the effects of transplantation matrix on the transplantation of A. roxburghii. [Results]MS + 0. 5 mg/L NAA + 2 mg/L BA + 20 g/L sucrose + 6 g/L agar was suitable for induction of stem segments of A. roxburghii; MS + 0. 5 mg/L NAA + 2 mg/L BA + 1 mg/L KT + 25 g/L sucrose + 6 g/L agar was most suitable for proliferation of cluster buds of A. roxburghii; MS + 1. 0 mg/L IBA + 1. 0 mg/L NAA + 1 g/L activated carbon + 50 g/L mashed banana + 25 g/L sucrose + 6 g/L agar was most suitable for rooting and seedling hardening of A. roxburghii; using peat soil: fine sand( 3∶ 1) as transplantation matrix,the survival rate was the highest. [Conclusions] The experiment results are expected to provide references for factory production of A. roxburghii.