Synergistic anticancer effect of green synthesized nickel nanoparticles and quercetin extracted from Ocimum sanctum leaf extract

作     者：Palanivel Rameshthangam Jeyaraj Pandian Chitra 

作者机构：Department of Biotechnology Science Campus Alagappa University Department of Biochemistry Manonmaniam Sundaranar University 

出 版 物：《Journal of Materials Science & Technology》 (材料科学技术（英文版）)

年 卷 期：2018年第34卷第3期

页      面：508-522页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 07[理学] 070205[理学-凝聚态物理] 08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 100602[医学-中西医结合临床] 10[医学] 0702[理学-物理学] 

主　　题：Nickel nanoparticles Quercetin Anticancer Apoptosis MCF 7 cells Ocimum sanctum 

摘      要：Leaf extract of medicinally important plant Ocimurn sanctum （O. sanctum） has been used for the synthesis of nickel nanoparticles （NiGs） and extraction of quercetin （Qu）. Qu has been conjugated with NiGs for enhanced anticancer effect on human breast cancer MCF-7 cells. Extracted Qu was conjugated with polyethylene glycol （PEG） coated NiGs （Qu-PEG-NiGs） which was used as carriers for breast cancer treatment. Anticancer activity of Qu-PEG-NiGs was evaluated by assessing cell viability, reactive oxygen species （ROS） production, caspase activity, mitochondrial membrane potential （MMP） and changes in nuclear morphology （staining methods）. 0.85 mg of quercetin was extracted from I g of leaves with retention time （Rt） of 2.914 rain. Loading and encapsulation efficiency of quercetin onto PEG-NiGs was 15.04% and 82% respectively and Qu-PEG-NiGs has shown a sustained release of Qu of about 84% after 48 h. Qu and Qu-PEG-NiGs showed dose dependent （1.56-50 μg/mL） anticancer effect against MCF-7 cells with IC50 values of 50 and 6.25 μg/mL respectively which was mediated by oxidative stress due to ROS over-production that induced loss of mitochondrial membrane potential, capsase -9, -7 activities leading to apoptosis. The present study validates that Qu-PEG-NiGs can be used as a potential anticancer agent for cancer therapy.