Neuroprotective mechanisms of rutin for spinal cord injury through anti-oxidation and anti-inflammation and inhibition of p38 mitogen activated protein kinase pathway

作     者：Hong-liang Song Xiang Zhang Wen-zhao Wang Rong-han Liu Kai Zhao Ming-yuan Liu Wei-ming Gong Bin Ning 

作者机构：Department of Spinal Surgery Jinan Central Hospital Affiliated to Shandong University Hospital Pharmacy Jinan Central Hospital Affiliated to Shandong University Department of Gynecology and Obstetrics Jinan Maternity and Child Care Hospital 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2018年第13卷第1期

页      面：128-134页

核心收录：

学科分类：10[医学] 

基　　金：supported in part by grants from the Young Scientists Awards Foundation of Shandong Province of China,No.BS2013YY049 the China Postdoctoral Science Foundation,No.2012M511036 

主　　题：nerve regeneration spinal cord injury rutin oxidative stress antioxidant anti-inflammation p38 mitogen activated protein kinase pathway anti-apoptosis caspase-3 caspase-9 neural regeneration 

摘      要：Rutin has anti-inflammatory, antioxidant, anti-viral, anti-tumor and immune regulatory effects. However, the neuroprotective effects of rutin in spinal cord injury are unknown. The p38 mitogen activated protein kinase （p38 MAPK） pathway is the most important member of the MAPK family that controls inflammation. We assumed that the mechanism of rutin in the repair of spinal cord injury is associated with the inhibition of p38 MAPK pathway. Allen’s method was used to establish a rat model of spinal cord injury. The rat model was intraperitoneally injected with rutin （30 mg/kg） for 3 days. After treatment with rutin, Basso, Beattie and Bresnahan locomotor function scores increased. Water content, tumor necrosis factor alpha, interleukin 1 beta, and interleukin 6 levels, p38 MAPK protein expression and caspase-3 and -9 activities in T8–9 spinal cord decreased. Oxidative stress related markers superoxide dismutase and glutathione peroxidase levels increased in peripheral blood. Rutin exerts neuroprotective effect through anti-oxidation, anti-inflammation, anti-apoptosis and inhibition of p38 MAPK pathway.