Histone 3 lysine 36 to methionine mutations stably interact with and sequester SDG8 in Arabidopsis thaliana

作     者：Guang Lin Ying Zhou Min Li Yuda Fang 

作者机构：National key Laboratory of Plant Molecular Genetics Chinese Academy of Sciences Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and Ecology Chinese Academy of Sciences University of Chinese Academy of Sciences Shanghai 200032 China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2018年第61卷第2期

页      面：225-234页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：supported by the National Key Research and Development Program of China (2016YFD0100902) Chinese Academy of Sciences (XDPB0403) 

主　　题：histone H3 lysine to methionine mutation SDG8 dominant negative effect Arabidopsis 

摘      要：Post-transcriptional modifications of histones play important roles in various biological processes. Here, we report that Arabidopsis plants overexpressing histone H3 lysine to methionine mutations at histone H3.1K36(H3.1K36M) and H3.3K36(H3.3K36M) have serious developmental defects with early-flowering and change in the modifications of endogenous histone H3, including acetylation at lysine 9(H3K9ac), trimethylation at lysine 27(H3K27me3), di-and tri-methylation at lysine 36(H3K36me2 and H3K36me3). In addition, H3K36M mutation alters its subcellular localization and interacts with H3K36 methyltransferase SDG8. Our results support a model in which H3K36M stably interacts with SDG8, and inhibits the activity of SDG8 by sequestering SDG8, resulting in a dominant negative effect to affect the proper expression levels of a variety of genes and plant development.