Toxicity testing of four silver nanoparticle-coated dental castings in 3-D LO2 cell cultures

作     者：Yi-ying ZHAO Qiang CHU Xu-er SHI Xiao-dong ZHENG Xiao-ting SHEN Yan-zhen ZHANG 

作者机构：Department of Food Science and Nutrition Zhejiang Key Laboratory for Agro-Food ProcessingFuli Institute of Food Science Huajiachi Dental Center Stomatology Hospital Affiliated to Zhejiang University of Medicine Department of General Dentistry the Second Affiliated Hospital School of Medicine Zhejiang University 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2018年第19卷第2期

页      面：159-167页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 0905[农学-畜牧学] 08[工学] 0906[农学-兽医学] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：Project supported by the Zhejiang Provincial Natural Science Foundation of China(No.LZ14C200001) the Public Welfare Project of Science Technology Department of Zhejiang Province(No.2013c33139),China 

主　　题：LO2 cell 3-D model Silver nanoparticles Dental alloys Toxicity test 

摘      要：To address the controversial issue of the toxicity of dental alloys and silver nanoparticles in medical ap- plications, an in vivo-like LO2 3-D model was constructed within polyvinylidene fluoride hollow fiber materials to mimic the microenvironment of liver tissue. The use of microscopy methods and the measurement of liver-specific functions optimized the model for best cell performances and also proved the superiority of the 3-D LO2 model when compared with the traditional monolayer model. Toxicity tests were conducted using the newly constructed model, finding that four dental castings coated with silver nanoparticles were toxic to human hepatocytes after cell viability assays. In general, the toxicity of both the castings and the coated silver nanoparticles aggravated as time increased, yet the nanoparticles attenuated the general toxicity by preventing metal ion release, especially at high concentrations.