Real-time Assessment of Cytosolic, Mitochondrial, and Nuclear Calcium Levels Change in Rat Pheochromocytoma Cells during Pulsed Microwave Exposure Using a Genetically Encoded Calcium Indicator
Real-time Assessment of Cytosolic, Mitochondrial, and Nuclear Calcium Levels Change in Rat Pheochromocytoma Cells during Pulsed Microwave Exposure Using a Genetically Encoded Calcium Indicator作者机构：Department of Experimental Pathology Beijing Institute of Radiation Medicine Beijing 100850 China Department of Radiation Protection and Health Physics Beijing Institute of Radiation Medicine Beijing 100850 China Department of General Surgery Shanghai Children's Hospital Shanghai Jiaotong University Shanghai 200040 China
出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)
年 卷 期：2017年第30卷第12期
页 面：927-931页
核心收录：
学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：supported by the National Natural Science Foundation of China[No.31400716 and No.61401497]
主 题：Real-time Assessment of Cytosolic Mitochondrial Nuclear Calcium Levels
摘 要：Little information is available about the effects of exposure to pulsed microwaves on neuronal Ca^2＋ signaling under non-thermal conditions. In this study, rat pheochromocytoma （PC12） cells were exposed to pulsed microwaves for 6 min at a specific absorption rate （SAR） of 4 W/kg to assess possible real-time effects. During microwave exposure, free calcium dynamics in the cytosol, mitochondria, and nucleus of cells were monitored by time-lapse microfluorimetry using a genetically encoded calcium indicator （ratiometric-pericam, ratiometric-10ericam-mt,
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