Expression of CXCL12-CXCR4 in osteosarcoma and its correlation with angiogenesis

作     者：Lu Han Yangyang Shen Wenhua Zhao Baoyong Sun Xin Zhang Kai Cui Lei Zhou Sheng Li 

作者机构：School of Medicine and Life Sciences University of Jinan-Shandong Academy of Medical Sciences Jinan 250117 China Department of Hepatobiliary Surgery Shandong Academy of Medical Sciences Shandong Cancer Hospital affiliated to Shandong University Jinan 250117 China Department of Anesthesiology Shandong Academy of Medical Sciences Shandong Cancer Hospital affiliated to Shandong University Jinan 250117 China Department of Tumor Minimally Invasive Surgery Qianfo Hill Hospital of Shandong Province Jinan 250014 China Department of Bone & Soft Tissue Surgery Shandong Academy of Medical Sciences Shandong Cancer Hospital affiliated to Shandong University Jinan 250117 China Shandong Institute of Pharmaceutical Research Jinan 250062 China Shandong Provincial Collaborative Innovation Center for Neurodegenerative Disorders Qingdao University Qingdao 266071 China 

出 版 物：《Oncology and Translational Medicine》 (肿瘤学与转化医学（英文版）)

年 卷 期：2017年第3卷第6期

页      面：254-259页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：osteosarcoma stromal cell-derived factor-1 (CXCL12) chemokine receptors-4 (CXCR4) angiogenesis pulmonary metastatics 

摘      要：Objective The expression of CXCL12(stromal cell-derived factor-1)-CXCR4(chemokine receptors-4) in osteosarcoma and its role in angiogenesis were *** The expression of CXCR4 and CXCL12 in 40 cases of osteosarcoma was detected by immunohistochemistry and real-time fluorescence quantitative *** expression of CD34 in osteosarcoma was detected by *** image analysis was performed to measure microvessel density(MVD).Additionally,the relationship between CXCL12 and CXCR4 expression and MVD of osteosarcoma and pulmonary metastasis were *** The positive rates of CXCL12 and CXCR4 protein expression in osteosarcoma were 40.0%(16/40) and 60.0%(24/40),*** quantitative real-time PCR indicated that the expression level of CXCR4 m RNA in pulmonary metastatic osteosarcoma was higher than that in nonpulmonary metastatic osteosarcoma(P 0.01).The level of MVD in pulmonary metastatic osteosarcoma was higher than that in non-pulmonary metastatic osteosarcoma(P 0.01).Conclusion The expression level of CXCR4 was significantly associated with pulmonary metastasis and angiogenesis of osteosarcoma.