Some pathogenic characters of paratyphoid Salmonella enterica strains isolated from poultry

作     者：Fabrizio Bertelloni Giovanni Tosi Paola Massi Laura Fiorentini Maria Parigi Domenico Cerri Valentina Virginia Ebani 

作者机构：Department of Veterinary ScienceUniversity of PisaViale Delle Piagge 256124 Pisa Italy Istituto Zooprofilattico Sperimentale Della Lombardia e Dell'Emilia RomagnaSezione Diagnostica di Forl(i)Via Don E. Servadei 3E/3F47122 Forl(i)(FC) Italy 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2017年第10卷第12期

页      面：1161-1166页

核心收录：

学科分类：090601[农学-基础兽医学] 1002[医学-临床医学] 09[农学] 0906[农学-兽医学] 

主　　题：Paratyphoid salmonellae Poultry Gastric acid resistance Virulence genes Antimicrobial resistance 

摘      要：Objective: To investigate some pathogenic characters of Salmonella enterica strains isolated from poultry.Methods: Twenty-three genetically distinct Salmonella enterica strains, of different serovars and pulsotype, were examined for virulence traits. Resistance to gastric acid environment was estimated by measuring the percentage of survived bacterial cells after exposure for 2 h to a synthetic gastric juice. Strains were analyzed with PCR for the presence of the following virulence genes: mgtC and rhu M located on SPI-3, sopB and pipB located on SPI-5, Salmonella virulence plasmid(spv)R(spvR), spvB and spvC located on Salmonella plasmid virulence and sodCI, sopE, and gipA located on prophage.Finally, resistance to 21 antibiotics was tested with Kirby–Bauer method.Results: A percentage of 82.60% of strains were resistant to gastric environment after induction and 60.87% of the strains exhibited constitutive resistance too. Nineteen different virulence profiles were detected. The phage related genes sodCI and sopE and the plasmid mediated operon spvR, spvB and spvC(spvRBC) were detected in 82.60%,47.82% and 52.17% of strains, respectively. Typhimurium and Enteritidis strains showed the highest number of virulence genes. Twenty-one different antibiotic resistance profiles were obtained and two isolates(Typhimurium and Enteritidis) resulted sensible to all the tested molecules. The ampicillin, streptomycin, sulfonamide and tetracycline resistance profile was detected in seven isolates(30.43%).Conclusion: Our results show that paratyphoid Salmonella strains with several characters of pathogenicity, that may be cause of severe pathology in animals and humans, are circulating among poultry.