Antibiofilm activity of a-mangostin extracted from Garcinia mangostana L. against Staphylococcus aureus

作     者：Nguyen Thi Mai Phuong Ngo Van Quang Ta Thu Mai Nguyen Vu Anh Chutima Kuhakarn Vichai Reutrakul Albert Bolhuis 

作者机构：Institute of BiotechnologyVietnam Academy of Science and Technology18 Hoang Quoc Viet RoadHanoiViet Nam Graduate University of Science and TechnologyVietnam Academy of Science and Technology18 Hoang Quoc Viet RoadHanoiViet Nam Institute of ChemistryVietnam Academy of Science and Technology18 Hoang Quoc Viet RoadHanoiViet Nam Center of Excellence for Innovation in Chemistry and Department of ChemistryFaculty of ScienceMahidol UniversityRama 6 RoadBangkok 10400Thailand Department of Pharmacy and PharmacologyUniversity of BathClaverton Down BathBA2 7AYBathUK 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2017年第10卷第12期

页      面：1154-1160页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by the TWAS research grant 14-062 RG/BIO/AS_G NAFOSTED grant 106-NN.02–2016.19 National Research Council of Thailand(NRCT)and Center of Excellence for Innovation in Chemistry(PERCH-CIC) 

主　　题：α-Mangostin Garcinia mangostana Biofilms Methicillin resistance Staphylococcus aureus 

摘      要：Objective: To isolate a-mangostin(AMG) from the peels of mangosteen(Garcinia mangostana L.), grown in Vietnam, and to investigate antibiofilm activity of this compound against three Staphylococcus aureus(S. aureus) strains, one of which was methicillin-resistant S. aureus(MRSA) and the other two strains were methicillinsensitive S. aureus(MSSA).Methods: AMG in n-hexane fraction was isolated on a silica gel column and chemically analyzed by HPLC and NMR. The antibiofilm activity of this compound was investigated by using a 96-well plate model for the formation of biofilms. Biofilm biomass was quantified using crystal violet. The viability of cells was observed under confocal microscopy using LIVE/DEAD Bac Light stains. Biofilm composition was determined using specific chemical and enzyme tests for polysaccharide, protein and DNA. Membranedamaging activity was assayed by measuring the hemolysis of human red blood cells in presence of ***: The results indicated that the isolated AMG, with a purity that exceeded 98%,had minimal inhibitory concentrations in the range of 4.6–9.2 mmol/L for the three strains tested. Interestingly, the MSSA strains were more sensitive to AMG than the MRSA strain. Minimal bactericidal concentrations were 2-fold higher than the minimal inhibitory concentration values for the three strains, indicating that AMG was a bactericidal compound. AMG also prevented biofilm formation effectively, albeit that again the MRSA strain was the most resistant. Interestingly, biofilms of the MRSA strain contained protein as a main component of the extracellular matrix, whereas this was polysaccharide in the MSSA strains. This might relate to the resistance of the MRSA 252 strain to *** using human red blood cells indicated that AMG caused significant membrane damage with 50% of cell lysis occurred at concentration of about 36 mmol/***: Our results provide evidence that the isolated AMG has inhibitory activity against biofilm formation