Molecular diversity analysis of Tetradium ruticarpum(Wu Zhu Yu) in China based on inter-primer binding site(iPBS) markers and inter-simple sequence repeat(ISSR) markers

作     者：XU Jing-Yuan ZHU Yan YI Ze WU Gang XIE Guo-Yong QIN Min-Jian 

作者机构：Department of Resources Science of Traditional Chinese Medicines State Key Laboratory of Natural Medicines China Pharmaceutical University 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2018年第16卷第1期

页      面：1-9页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the National Science and Technology Major Projects for"Major New Drugs Innovation and Development" and the"Chinese Herbal Medicine Seeds and Seedlings Planting(breeding)Standard Platform Topics"(No.2012ZX09304006) 

主　　题：Genetic diversity Tetradium ruticarpum iPBS Retrotransposon ISSR 

摘      要：Wu zhu yu, which is obtained from the dried unripe fruits of Tetradium ruticarpum(A. Jussieu) T. G. Hartley, has been used as a traditional Chinese medicine for treatment of headaches, abdominal colic, and hypertension for thousands of years. The present study was designed to assess the molecular genetic diversity among 25 collected accessions of T. ruticarpum(Wu zhu yu in Chinese) from different areas of China, based on inter-primer binding site(i PBS) markers and inter-simple sequence repeat(ISSR) markers. Thirteen ISSR primers generated 151 amplification bands, of which 130 were polymorphic. Out of 165 bands that were amplified using 10 iPBS primers, 152 were polymorphic. The iPBS markers displayed a higher proportion of polymorphic loci(PPL = 92.5%) than the ISSR markers(PPL = 84.9%). The results showed that T. ruticarpum possessed high loci polymorphism and genetic differentiation occurred in this plant. The combined data of iPBS and ISSR markers scored on 25 accessions produced five clusters that approximately matched the geographic distribution of the species. The results indicated that both iPBS and ISSR markers were reliable and effective tools for analyzing the genetic diversity in T. ruticarpum.