Effects of Total Flavonoids ofHippophae *** Intracellular Free Calciumin Cultured Vascular Smooth Muscle Cells of Spontaneously Hypertensive Rats and Wistar-Kyoto Rats
Effects of Total Flavonoids of Hippophae Rhamnoides L. on Intracellular Free Calcium in Cultured Vascular Smooth Muscle Cells of Spontaneously Hypertensive Rats and Wistar-Kyoto Rats作者机构:Cardiovascular Depart ment Nanhui District CentralHospital Shanghai 201300 Cardiovascular Depart-ment Shanghai Renhe Hospital Internal Medicine De-part ment Longhua Hospital Shanghai University of Tradi-tional Chinese Medicine
出 版 物:《Chinese Journal of Integrative Medicine》 (中国结合医学杂志)
年 卷 期:2005年第11卷第4期
页 面:287-292页
学科分类:1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
基 金:Supported by One-hundred-people Plan of Hygiene Systemin Shanghai (No .990122)
主 题:total flavonoids of Hippophae rhamnoides L. spontaneously hypertensive rats Wistar-Kyoto rats vascular smooth muscle cells intracellular free calcium
摘 要:Objective: To explore the effects of total flavonoids of Hippophae rhamnoides L. (TFH), quercetin (Que) and isorhamnetin (Isor) on the intracellular free calcium (Ca~ 2+ _ i) in vascular smooth muscle cells (VSMC) of spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY).Methods: Fluo 3-acetoxymethylester(Fluo-3/AM) was used to observe the effects of TFH (100mg/L) and its essential monomers, namely Que (10~ -4 mol/L) and Isor (10~ -4 mol/L) on changes of Ca~ 2+ _ i in cultured SHR and WKY VSMC (abbr. to Ca-SHR & Ca-WKY) following exposure to high K~+, norepinephrine (NE) and angiotensin Ⅱ(AngⅡ), and to compare with the effects of verapamil (Ver). Results: (1) TFH, Que and Isor had inhibitory effects on resting Ca-SHR (P0.05). (2) High K~+ could increase Ca-SHR more significantly than Ca-WKY (P0.05); TFH, Que and Isor could inhibit the elevation of Ca~ 2+ _ i induced by high K~+-depolarization, with the effects similar to that of Ver, and the effect on Ca-SHR was more significant than that on Ca-WKY (P0.05). (3) NE and AngⅡcould increase Ca-SHR more significantly than Ca-WKY (P0.05), TFH, Que and Isor had remarkably inhibitory effect on the elevation of Ca-SHR and Ca-WKY induced by NE or AngⅡ. (4) In the absence of extracellular Ca~ 2+ , TFH, Que and Isor also had certain inhibitory effect on Ca-SHR and Ca-WKY induced by NE, and the effect on the former was more significant than that on the latter(P0.05). Conclusion: TFH, Que and Isor might decrease the levels of Ca~ 2+ _ i in VSMCs by blocking both voltage-dependent calcium channels (VDC) and receptor-operated calcium channels (ROC) in physiological or pathological state, which may be one of the important mechanisms of their hypotensive and protective effects on target organs in patients with hypertension.