Tannin inhibits HIV-1 entry by targeting gp41

作     者：Lin Lü2, Shu-wen LIU2,3, Shi-bo JIANG3, Shu-guang WU2,4 2Institute of Pharmaceutical Sciences of the First Medical University of PLA, Key Lab of GD for Screening of New Drug, Guangzhou, Guangdong 510515, China Lindsley F Kimball Research Institute, New York Blood Center, New York, NY 10021, USA 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2004年第2期

页      面：87-92页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：This project was supported by the National High Technology Foundation of China (“863” Project: 2001AA214201) National Natural Science Foundation of China (301400220) Chinese Army Medical Science and Technology Foundation (01Z051) Guangdong P 

主　　题：tannin drug dose-response relationship enzyme-linked immunosorbent assay HIV envelope pro- tein gp41 phenols plant extracts medical plants 

摘      要：AIM: To investigate the mechanism by which tannin inhibits HIV-1 entry into target cells. METHODS: The inhibitory activity of tannin on HIV-1 replication and entry was detected by p24 production and HIV-1-mediated cell fusion, respectively. The inhibitory activity on the gp41 six-helix bundle formation was determined by an improved sandwich ELISA. RESULTS: Tannins from different sources showed potent inhibitory activity on HIV-1 replication, HIV-1-mediated cell fusion, and the gp41 six-helix bundle formation. CONCLUSION: Tannin inhibits HIV-1 entry into target cells by interfering with the gp41 six-helix bundle formation, thus blocking HIV-1 fusion with the target cell.