Altered gene expression profiles of NIH3T3 cells regulated by human lung cancer associated gene CT120
Altered gene expression profiles of NIH3T3 cells regulated by human lung cancer associated gene CT120作者机构：State Key Laboratory of Oncogenes and Related Genes Shanghai Cancer Institute Shanghai Jiao-Tong University Medical School 2200Ln/25 Xie Tu Road Shanghai 200032 China
出 版 物：《Cell Research》 (细胞研究(英文版))
年 卷 期：2004年第14卷第6期
页 面：487-496页
核心收录：
学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：国家重点基础研究发展计划(973计划)(2004CB518704and2002CB513100)
主 题：CT120 cell proliferation lung gene expression metastasis.
摘 要：CT120, a novel membrane-associated gene implicated in lung carcinogenesis, was previously identified from chromosome 17p13.3 locus, a hot mutation spot involved in human malignancies. In the present study, we further determined that CT120 ectopic expression could promote cell proliferation activity of NIH3T3 cells using MTS assay, and monitored the downstream effects of CT120 in NIH3T3 cells with Atlas mouse cDNA expression arrays. Among 588 known genes, 133 genes were found to be upregulated or downregulated by CT120. Two major signaling pathways involved in cell proliferation, cell survival and anti-apoptosis were overexpressed and activated in response to CT120: One is the Raf/MEK/Erk signal cascades and the other is the PI3K/Akt signal cascades, suggesting that CT120 might contribute, at least in part, to the constitutively activation of Erk and Akt in human lung caner cells. In addition, some tumor metastasis associated genes cathepsin B, cathepsin D, cathepsin L, MMP-2/TIMP-2 were also upregulated
同方期刊数据库