Protective Effects of Traditional Tibetan Medicine Zuo-Mu-A Decoction(佐木阿汤)on the Blood Parameters and Myocardium of High Altitude Polycythemia Model Rats

作     者：LU Meng-qian Nyima Tsring YU Tian-yuan WU Jian-cong Steven Wong CHEN Guo-yong Pasang Dekyi PAN Fan XIAN Si-tong Dorje Rinchen MAO Ying-qiu ZHANG Lin-feng YAO Bin-bin 

作者机构：School of Acupuncture-Moxibustion and TuinaBeijing University of Chinese MedicineBeijing 100029China Tibetan Traditional Medical CollegeLhasa 850000China Department of TuinaBeijing Massage HospitalBeijing 100035China Information CenterBeijing University of Chinese MedicineBeijing 100029China Tibet Academy of Tibetan MedicineLhasa 850000China Center of Scientific ExperimentBeijing University of Chinese MedicineBeijing 100029China 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2017年第23卷第12期

页      面：908-915页

核心收录：

学科分类：1006[医学-中西医结合] 1005[医学-中医学] 100513[医学-民族医学(含：藏医学、蒙医学等)] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by the National Natural Science Foundation of China(No.81260567) 

主　　题：erythropoietin hematology myocardium high altitude polycythemia traditional medicine 

摘      要：Objective: To explore the protective effects of Tibetan medicine Zuo-Mu-A Decoction(佐木阿汤, ZMAD) on the blood parameters and myocardium of high altitude polycythemia(HAPC) model rats. Methods: Forty male Wistar rats were randomly divided into 4 groups by a random number table, including the normal, model, Rhodiola rosea L.(RRL) and ZMAD groups(10 in each group). Every group was raised in Lhasa to create a HAPC model except the normal group. After modeling, rats in the RRL and the ZMAD groups were administered intragastrically with RRL(20 mL/kg) and ZMAD(7.5 mL/kg) once a day for 2 months, respectively; for the normal and the model groups, 5 mL of distilled water was administered intragastrically instead of decoction. Then routine blood and hematologic rheology parameters were taken, levels of erythropoietin and 8-hydroxy-2’-deoxyguanosine(8-OHd G) were tested, and ultrastructural change in the left ventricular myocardium was observed using transmission electron microscopy. Results: Compared with the model group, ZMAD significantly reduced the red blood cell count, hemoglobin levels, whole blood viscosity at low/middle shear rates, plasma viscosity, erythrocyte electrophoretic time, erythropoietin and 8-OHd G levels, and also increased the erythrocyte deformation index(P0.05). There was no difference in all results between the RRL and the ZMAD groups. The cardiac muscle fibers were well-protected, mitochondrial matrix swelled mildly and ultrastructure changes were less prominent in the ZMAD group compared with the model group. Conclusion: ZMAD has significant protective effects on the blood parameters against HAPC, and also has the beneficial effect in protecting against myocardial injury.