A synthetic biological secondary metabolite,Lycogen^TM,produced and extracted from Rhodobacter sphaeroides WL-APD911 in an optimizatioal scale-up strategy

作     者：Cheng-Chin Wang Shi-Ying Huang Shu-Hung Huang Zhi-Hong Wen Jyun-Ying Huang Wen-Sheng Liu Hui-Min David Wang 

作者机构：Research and Development DepartmentChia-Yi SteelTainan 717TaiwanChina College of Oceanology and Food ScienceQuanzhou Normal UniversityQuanzhou 362000China Fujian Province Key Laboratory for the Development of Bioactive Material from Marine AlgaeQuanzhou 362000China Division of Plastic SurgeryDepartment of SurgeryKaohsiung Medical University HospitalKaohsiung Medical UniversityKaohsiung 807TaiwanChina Graduate Institute of MedicineCollege of MedicineKaohsiung Medical UniversityKaohsiung 807TaiwanChina Center for Stem Cell ResearchKaohsiung Medical UniversityKaohsiung 807TaiwanChina Department of SurgeryFaculty of MedicineCollege of MedicineKaohsiung Medical UniversityKaohsiung 807TaiwanChina Orthopaedic Research CenterKaohsiung Medical UniversityKaohsiung 807TaiwanChina Doctoral Degree Program in Marine BiotechnologyNational Sun Yat-sen University and Academia SinicaKaohsiung 804TaiwanChina Marine Biomedical Laboratory and Center for Translational BiopharmaceuticalsDepartment of Marine Biotechnology and ResourcesNational Sun Yat-sen UniversityKaohsiung 804TaiwanChina Graduate Institute of Biomedical EngineeringNational Chung Hsing UniversityTaichung 402TaiwanChina Department and Graduate Institute of AquacultureNational Kaohsiung Marine UniversityKaohsiung 811TaiwanChina Asia-Pacific Biotech DevelopingInc.Kaohsiung 803TaiwanChina Department of Medical ResearchChina Medical University HospitalChina Medical UniversityTaichung 404TaiwanChina 

出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康（英文）)

年 卷 期：2017年第6卷第4期

页      面：195-201页

核心收录：

学科分类：081703[工学-生物化工] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 0836[工学-生物工程] 082203[工学-发酵工程] 0822[工学-轻工技术与工程] 

基　　金：This work was supported by grants from the Ministry of Science and Technology Taiwan ROC(MOST 104-2221-E-005-096-MY2 and MOST 104-2628-E-005-004-MY3).We thank the projects of Center for Stem Cell Research Kaohsiung Medical University Kaohsiung Taiwan KMU-TP104G00 and KMU-TP104G02-05.The financial supports were also from KMU-DK105005 and NSYSUKMU105-P 007 

主　　题：Rhodobacter sphaeroides WL-APD911 Lycogen^TM Response surface methodology(RSM) Ferementation 

摘      要：The optimization of fermentation medium is important for synthetic biological secondary metabolite *** effect of rotation speed,inoculum amount,and medium supplements on the cell growth and Lycogen^TM secretion of photobacterium Rhodobacter sphaeroides WL-APD911 was *** results reveal that a higher rotational speed exhibit a higher cell density,and the increasing in the amount of inoculum amount show a slight augment on the growth of *** *** the case of nitrogen sources adding,Lycogen^TM production was achieved with a 0.5 mM l-lysine ***,the attention of Tween 80 presented a tremendous increase in the secondary *** surface methodology(RSM)exhibited the optimization of medium supplements for Lycogen^TM invention is accomplished at molasses concentration of 10 g/L,yeast extract concentration of 40 g/L,0.3%Tween 80 and NaCl concentration of 5 g/L,***,the batch fermentation is carried out in both 5 L and 20 L fermentors to study the scale-up process factors to be *** a 20 L fermentor,Lycogen^TM yields under the optimal culture condition are over 2 times than in the shake *** present results provide the Lycogen^TM optimal culture mediums,scale-up procedures and efficient extractions from *** WL-APD911.