The role of CopG mediated DNA bending on the regulation of the σ^(54)-dependent promoters in E. coli

作     者：YANG Ence 

作者机构：National Laboratory of Protein Engineering and Plant Genetic Engineer- ing College of Life Sciences Peking University 

出 版 物：《Chinese Science Bulletin》 (CHINESE SCIENCE BULLETIN)

年 卷 期：2006年第51卷第8期

页      面：934-940页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 

基　　金：supported in part by the National Basic Research Program of China(Grant No.2001CB 108903) National Natural Science Foundation of China(Grants No.39925017&30200047) 

主　　题：DNA CopG 启动子 大肠杆菌 

摘      要：In order to investigate the role of DNA bending on the regulation of σ54-dependent promot- ers, we introduced the CopG binding site between the enhancer-like element and the core promoter of glnAp2, nifLp and glnHp2, without changing the dis- tance in between. The expression activities of these homologous promoters were either activated or re- pressed by the CopG-induced DNA bending in E. coli. In this case, similar regulatory pattern (either acti- vated or repressed) could be observed, when the bending centers from CopG are in integral DNA he- lixes interval, while opposite regulatory pattern could be observed, when the bending centers from CopG are in integral plus a half DNA helixes interval. These results suggested that CopG-induced DNA bending can exert regulatory effects on the transcription of σ54-dependent promoters probably by altering the relative DNA helix phase of 54 RNA polymerase and NtrC.