Single-molecule analysis in an electrochemical confined space

作     者：Yi-Lun Ying Yi-Tao Long 

作者机构：Key Laboratory for Advanced Materials&School of Chemistry and Molecular EngineeringEast China University of Science and TechnologyShanghai 200237China 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2017年第60卷第9期

页      面：1187-1190页

核心收录：

学科分类：07[理学] 070302[理学-分析化学] 0703[理学-化学] 

基　　金：supported by the National Natural Science Foundation of China(21421004,21505043,21327807) the Fundamental Research Funds for the Central Universities(222201718001,222201717003,222201714012) 

主　　题：electrochemical confined space, nanopores, single-molecule analysis, DNAs 

摘      要：Electrochemical analysis of single molecules is a method with the strong ability of the enhanced efficiency and ultra-sensitivity.Here,we demonstrate that the electrochemical confined space could efficiently convert single molecule characteristics into measurable electrochemical signatures with high temporal resolution.The human telomere repeat sequence T8 was used as a probe to determine the electrochemical confined effect in a nanopore.Our results show that the nanopore with comparable confined space of the telomere repeat sequence exhibits the most distinguishable single-molecule signals which suggest the folded conformation of T8.This method will greatly extend the lifetime of a metastable conformation for a single biomolecule by strong analyte-nanopore interactions,which brings the new insight into the understanding of the biomolecule s function at single-molecule level.