Effects of Pinealectomy and Gonadectomy on Olfactory Bulb Dopaminergic Neurons in Rats

作     者：Yan Li Jian Zhu Ying Wang Lei Guo3, Lei Li Dong Wang Li Yan;Zhu Jian;Wang Ying;Guo Lei;Li Lei;Wang Dong

作者机构：Department of Neurology Jinan Central Hospital Affiliated to Shandong University Jinan Shandong 250013 China Department of Medical Imaging Shandong Provincial Hospital Jinan Shandong 250014 China Department of Medical Imaging Jinan Central Hospital Affiliated to Shandong University Jinan Shandong 250013 China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2017年第130卷第19期

页      面：2302-2306页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 071002[理学-动物学] 090102[农学-作物遗传育种] 

主　　题：Apoptosis Gonadectomy Olfactory Bulb Pinealectomy Tyrosine Hydroxylase 

摘      要：Background： Olfactory disorder is an early manifestation of Parkinson＆#39;s disease （PD）, likely to be associated with abnormalities of the dopaminergic neurons in the olfactory bulb （OB）; however, the causes of olfactory disorder in PD are not entirely clear. Some studies showed that melatonin （MT） and androgens （mainly testosterone, T） might participate in the pathogenesis of PD. The research aimed to investigate effects of MT or T deficiency on OB dopaminergic neurons in rats. Methods： One hundred and twenty normal male Wistar rats were randomly divided into the control, sham operation pinealectomy （PX）, sham operation gonadectomy （GDX）, PX, GDX, and PX ＋ GDX groups. After 60 days, glial cell hyperplasia and neuronal apoptosis were examined with hematoxylin and eosin and the TUNEL method; the expression levels of tyrosine hydroxylase （TH）, Bax, and Bcl-2 were measured using immunohistochemistry （IH） by the streptavidin peroxidase conjugated method. Comparison among multiple sets used analysis of variance and LSD method or Kruskal-Wallis test and Nemenyi method. Results： There were no significant differences between the sham operation groups and the control group; thus, they were merged into Group A. There was no significant glial cell hyperplasia （P 〉 0.05） or change in shape in any of the groups after PX or GDX. The number of apoptotic cells in Groups A （1.41 ± 0.56）, PX （12.31 ± 4.68）, GDX （20.52 ± 5.13）, and PX ＋ GDX （30.23 ± 5.25） successively significantly increased （P 〈 0.05）. The number of TH （＋） cells in Groups A （42.62 ± 5.63）, PX （37.31 ± 4.32）, GDX （31.07 ± 4.21）, and PX ＋ GDX （25.22 ± 3.66） was successively significantly decreased （P 〈 0.05）. The gray value of TH （＋） cells and fibers in Groups A （98.51 ± 10.36）, PX （108.96 ± 13.01）, GDX （119.02 ± 12.98）, and PX ＋ GDX （128.99 ± 13.39） was successively significantly increased （P 〈 0.05）. The results of Bax staining were as follows： Group A＋, Group PX＋＋, Group GDX＋＋, and Group PX＋ GDX＋＋＋, the results of