Expression of p53, p16 and COX-2 in pancreatic cancer with tissue microarray

作     者：Lei Xu, You-Ming Li, Chao-Hui Yu, Lan Li, You-Shi Liu, Bao-Feng Zhang, Jing Fang, Qiong Zhou, Ying Hu and Hen-Jun Gao Department of Gastroenterology, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China and National Engineering Center for Biochip, Shanghai 200000, China 

作者机构：Department of Gastroenterology First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China. 

出 版 物：《Hepatobiliary & Pancreatic Diseases International》 (国际肝胆胰疾病杂志（英文版）)

年 卷 期：2006年第5卷第1期

页      面：138-142页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：The study was supported by a grant from the National 863 program (2002AA2Z2021) 

主　　题：pancreatic cancer tissue microarray p53 p16 cyclooxygenase-2 

摘      要：BACKGROUND: Pancreatic cancer development and progression is driven by the accumulation of genetic changes. In this study we constructed tissue microarray containing specimens from pancreatic cancer, adjacent non-cancer tissue and normal tissue to survey the expression of p53, p16 and cyclooxyganase-2 (COX-2). METHODS: Tissue microarray containing 337 specimens from different stages of pancreatic cancer, adjacent noncancer tissue and normal tissues was constructed, and the expression of p53, p16 and COX-2 was assayed by immunohistochemistry to consecutive formalin-fixed tissue microarray sections. RESULTS: The expression of p53, p16 and COX-2 was significantly higher in tumorous tissues than in non-tumorons ones. A significant relationship was observed between p53 and COX-2, or p16 and COX-2. But no obvious correlation was seen between p53 and p16 expressions. Logistic regression analysis showed p53 and COX-2 as dependent predictors in pancreatic carcinogenesis, and a reciprocal relationship to neoplastic progression between p53 and COX-2. CONCLUSION: Combination analysis of p53 and COX-2 may be useful in predicting pancreatic carcinogenesis.