In vitro neuroprotective effects of ciliary neurotrophic factor on dorsal root ganglion neurons with glutamate-induced neurotoxicity

作     者：Shu-yun Wen Ai-min Li Kuan-qing Mi Rui-zheng Wang Hao Li Hua-xiang Liu Yi Xing 

作者机构：Department of NeurosurgeryShandong Provincial Hospital Affiliated to Shandong UniversityJinanShandong ProvinceChina Department of RheumatologyQilu HospitalShandong UniversityJinanShandong ProvinceChina Department of RheumatologyQingdao Fifth People’s HospitalQingdaoShandong ProvinceChina Department of NeurosurgeryJinan Fifth People's HospitalJinanShandong ProvinceChina Department of OrthopedicsQilu HospitalShandong UniversityJinanShandong ProvinceChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2017年第12卷第10期

页      面：1716-1723页

核心收录：

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

基　　金：supported by the Natural Science Foundation of Shandong Province of China,No.ZR2014HQ065 a grant from the Medical Science and Technology Development Project of Shandong Province of China,No.2015WS0445 

主　　题：nerve regeneration ciliary neurotrophic factor JAK2/STAT3 PI3K/Akt glutamate neuron excitotoxicity neuroprotection growth associated protein 43 neurite outgrowth dorsal root ganglion neural regeneration 

摘      要：Ciliary neurotrophic factor has neuroprotective effects mediated through signal transducer and Janus kinase（JAK） 2/activator of transcription 3（STAT3） and phosphatidylinositol 3-kinase（PI3 K）/Akt signaling *** ciliary neurotrophic factor is neuroprotective for glutamate-induced excitotoxicity of dorsal root ganglion neurons is poorly *** the present study,the in vitro neuroprotective effects of ciliary neurotrophic factor against glutamate-induced excitotoxicity were determined in a primary culture of dorsal root ganglion neurons from Wistar rat embryos at embryonic day *** the JAK2/STAT3 and PI3 K/Akt signaling pathways were related to the protective effects of ciliary neurotrophic factor was also *** exposure inhibited neurite outgrowth,cell viability,and growth-associated protein 43 expression and promoted apoptotic neuronal cell death,all of which were reversed by the administration of exogenous ciliary neurotrophic ***,preincubation with either JAK2 inhibitor AG490 or PI3 K inhibitor LY294002 blocked the neuroprotective effect of ciliary neurotrophic *** data indicate that the two pathways JAK2/STAT3 and PI3 K/Akt play major roles in mediating the in vitro neuroprotective effects of ciliary neurotrophic factor on dorsal root ganglion neurons with glutamate-induced neurotoxicity.