Determination of thyreostats in bovine urine using ultra-high performance liquid chromatography-tandem mass spectrometry

作     者：Lech RODZIEWICZ Jolanta MASLOWIECKA Anna SADOWSKA Halina CAR 

作者机构：Laboratory of Chemical Examination of FoodstuffsDepartment of Veterinary Hygiene Department of Experimental PharmacologyMedical University of Bialystok 

出 版 物：《色谱》 (Chinese Journal of Chromatography)

年 卷 期：2017年第35卷第10期

页      面：1048-1054页

核心收录：

学科分类：0710[理学-生物学] 081704[工学-应用化学] 07[理学] 0817[工学-化学工程与技术] 08[工学] 070302[理学-分析化学] 0703[理学-化学] 

主　　题：ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) thyreostats (TSs) urine 

摘      要：Five thyreostats(TSs),namely tapazole,thiouracil,methylthiouracil,propylthiouracil,and phenylthiouracil,were determined in bovine urine using ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)in positive electrospray ionization *** and clean-up were achieved using a ChemElut cartridge with tert-butyl methyl ether,without a derivatization *** was achieved on an Acquity UPLC SS T3 *** mobile phase was acetonitrile and water containing 0.2%(v/v)formic *** mass spectrometer was operated in multiple reaction monitoring *** samples were spiked with TS solution at levels corresponding to 5,10,15,and 20μg/*** accuracy(internal standard corrected)ranged from 92%to 107%,with a repeatability precision(relative standard deviation,RSD)less than 15%for all five *** RSDs within-laboratory reproducibility was less than 26%.The decision limits(CCα)and detection capabilities(CCβ)were obtained from a calibration curve and were in the ranges of 3.1-6.1μg/L and 4.0-7.4μg/L,*** CCαand CCβvalues were below the recommended concentration,which was set at 10μg/*** results show that the described method is suitable for the direct detection of TSs in bovine *** method can also be used to determine TSs in porcine urine.