Effect of differential rearing environments on nicotine-stimulated locomotor activity and nicotinic acetylcholine receptor subtypes

作     者：CS BOCKMAN M QUAST DJ STAIRS 

作者机构：Department of PharmacologyCreighton University School of MedicineCreighton University School of Arts & Sciences Department of PsychologyCreighton University School of Arts & Sciences 

出 版 物：《中国药理学与毒理学杂志》 (Chinese Journal of Pharmacology and Toxicology)

年 卷 期：2017年第31卷第10期

页      面：1014-1014页

核心收录：

学科分类：1002[医学-临床医学] 100205[医学-精神病与精神卫生学] 10[医学] 

基　　金：supported by Nebraska Cancer and Smoking Disease Research Programs LB506and LB595 to CS BOCKMAN and DJ STAIRS 

主　　题：nicotine addiction environmental enrichment α4β2*-nicotinic acetylcholine receptor α7-nicotinic acetylcholine receptor 

摘      要：OBJECTIVE Individuals vary in sensitivity to the behavioral effects of nicotine,resulting in differences in their vulnerability to *** role of rearing environment in determining individual sensitivity to nicotine is *** neuropharmacological mechanisms mediating the effect of rearing environment on the actions of nicotine are also ***,the contribution of rearing environment in determining the sensitivity to the locomotor effects of nicotine and regulating α4β2*-and α7-nicotinic acetylcholine(n ACh) receptor expressionwas determined in rats reared in isolated(IC) or enriched(EC) *** To measure locomotor activity,adolescent rats(postnatal day 21-51)were injected with saline(1 mL·kg^(-1)) or nicotine(0.3 mg·kg^(-1)) subcutaneously,then placed in chamberswhere ambulatory activity was monitored for 30-min by computer for 14 daily sessions.α4β2*-andα7-n ACh receptor expression in the mesolimbic dopamine pathway was determined by quantitative autoradiography of [125 I]-epibatidine and [125 I]-bungarotoxinbinding,respectively,in 16 μmol·L^(-1) coronal *** for receptor expression in fmol are ±s of 8 brains and compared by two-tailed,unpaired t-test with P0.05 considered *** EC-rats are similarly sensitive as IC-rats to the locomotor effects of nicotine.[125 I]-epibatidine binding in the ventral tegmental area of EC-rats was reduced(2.8±0.3 fmo L) compared to IC-rats(4.0±0.4 fmo L);there was no difference in the nucleus *** was no difference between EC-and IC-rats in α7-n ACh receptor expression in the mesolimbic dopamine *** Rearing environment differentially regulates n ACh receptor subtypes in EC and IC *** data suggest regulation of n ACh receptors by environmental factors may be a mechanism for the protective effect of enrichment against altered sensitivity to nicotine in genetically vulnerable *** characterization of these mechanisms will aid in develo