Effect of Xiaoqinglong decoction on mucus hypersecretion in the airways and cilia function in a murine model of asthma

作     者：Mingyue Qi Jichao Zhou Xiaowei Zhang Xianggen Zhong Yuping Zhang Xiaojing Zhang Xiulan Deng Haiyan Li Qingqing Wang 

作者机构：Beijing University of Chinese MedicineBeijing 100029China Molecular Immunology and Pharmacology GroupState Key Laboratory of Bioactive Substances and Functions of Natural MedicinesInstitute of Materia MedicaChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China Department of Internal NeurologyWeihaiwei People's HospitalWeihai 26400China 

出 版 物：《Journal of Traditional Chinese Medical Sciences》 (中医科学杂志（英文）)

年 卷 期：2017年第4卷第3期

页      面：290-296页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：This study was supported by the National Natural Science Foundation of China(81403313) 

主　　题：Asthma Xiaoqinglong decoction Mucus Ciliophagy IL-13 

摘      要：Objective:Xiaoqinglong decoction (XQL) has been used in traditional Chinese medicine as a prescription for asthma *** explored the effects of XQL on mucus hypersecretion and ciliophagy in the airways of mice in which asthma had been induced by ovalbumin (OVA).Methods:Thirty-six mice were sensitized by OVA injection (i.p.) on day-0 and day-14 and challenged with 1% OVA on day ***,they were divided into three groups:model,carbocysteine and *** day-15 to day-22,the XQL group was administered XQL (10 g/kg,p.o.) 1 hour before each aerosol challenge with *** evaluate the effect of XQL on mucus hypersecretion,AB-PAS staining,measurement of serum levels of interleukin (IL)-13,bronchoalveolar lavage fluid (BALF) analyses,ciliophagy analyses,as well as coexpression of Light Chain 3 (LC3) and acetylated α-tubulin by immunofluorescence staining were ***:Treatment with XQL (10 g/kg) attenuated mucus secretion in the airways,and reduced the positive areas of AB-PAS staining in histopathologic lung tissues (P .05).IL-13 expression in serum (P .01),OVA-induced inflammatory changes,and the number of white blood cells (P .01) in BALF samples were also ***,the effect on mucus secretion was less apparent in the carbocysteine group compared with the XQL *** treatment also improved the cilia length and elicited a substantial reduction in ciliophagy and LC3 expression in the tracheal ***:XQL can attenuate cilia shortening,aid the clearance function of ciliated epithelial cells,and reduce mucus production in an OVA-induced asthma model in *** can inhibit mucus hypersecretion and could be a new type of pharmacotherapy.