Morphological and ISSR molecular markers reveal genetic diversity of wild hawthorns (Crataegus songorica K. Koch.) in Xinjiang, China

作     者：SHENG Fang CHEN Shu-ying TIAN Jia LI Peng QIN Xue WANG Lei LUO Shu-ping LI Jiang 

作者机构：College of Forestry and Horticulture Xinjiang Agricultural University Urumqi 830052 P.R. China Academy of Forestry in Ili Yining 835000 P.R. China Zhengzhou Fruit Research Institute Chinese Academy of Agricultural Sciences Zhengzhou 450009 P.R. China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2017年第16卷第11期

页      面：2482-2495页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：supported by the Special Research Projects of National Forestry Industry of Public Benefit,China(201304701-1) the Key Discipline of Horticultural of Xinjiang Uygur Autonomous Region of China(2016-10758-3) the Key Laboratory of Characteristics of Fruit Trees Center of Xinjiang Agricultural University,China 

主　　题：phenotypic traits ISSR marker genetic diversity Crataegus songorica K. Koch. germplasm resources molecular marker 

摘      要：The wild hawthorn species, Crataegus songorica K. Koch., is an important wild germplasm resource in Xinjiang, China that has been endangered in recent years. The genetic diversity of C. songorica K. Koch. germplasm in five populations from Daxigou, Xinjiang, China were evaluated based on phenotypic traits and ISSR molecular markers to provide basic infor- mation on resource protection, rational utilization and genetic improvement. The F-value for the phenotypic differentiation coefficient of the 33 traits measured ranged from 0.266 to 15.128, and mean value was 13.85%. The variation among populations was found to be lower than that within population. A total of 303 loci were detected within the five populations by 12 primers. Within 298,polymorphic loci, the polymorphism was 98.35%, showing a high genetic diversity in C. songorica K. Koch. The gene diversity within population, total population genetic diversity, genetic differentiation coefficient and gene flow were 0.2779, 0.3235, 0.1408, and 3.0511, respectively. Our results showed that C. songorica K. Koch. from Xinjiang has a high level of genetic diversity at both the phenotypic and molecular levels. Significant genetic differentiation existed within population and the differentiation trend showed a regional association. And in this study, in situ and ex situ conser- vation approaches were raised for wild hawthorn protection utilization.