Heterologous biosynthesis of natural product naringenin by co-culture engineering

作     者：Vijaydev Ganesan Zhenghong Li Xiaonan Wang Haoran Zhang 

作者机构：Department of Chemical and Biochemical EngineeringRutgersThe State University of New Jersey98 Brett RdPiscatawayNJ 08854USA 

出 版 物：《Synthetic and Systems Biotechnology》 (合成和系统生物技术（英文）)

年 卷 期：2017年第2卷第3期

页      面：236-242页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070305[理学-高分子化学与物理] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 

基　　金：The authors gratefully acknowledge Professor Matthews Koffas(Rensselaer Polytechnic Institute)and Professor Gregory Stephanopoulos(Massachusetts Institute of Technology)for the generous gift of plasmids pOM-PhCHS-MsCHI pCDF-trc-RgTAL^syn-Pc4CL^syn and pCA1 in support of this study.This work is supported by startup research funds from Rutgers The State University of New Jersey.Zhenghong Li is grateful for the Ph.D.fellowship from the China Scholarship Council 

主　　题：Co-culture engineering Naringenin Heterologous biosynthesis E.coli 

摘      要：Co-culture engineering is an emerging approach for microbial biosynthesis of a variety of *** this study,*** co-cultures were developed for heterologous biosynthesis of the natural product *** co-cultures were composed of two independent *** strains dedicated to functional expression of different portions of the biosynthetic pathway,*** co-culture biosynthesis was optimized by investigating the effect of carbon source,*** strain selection,timing of IPTG induction and the inoculation ratio between the co-culture *** with the monoculture strategy,the utilization of the designed co-cultures significantly improved the naringenin production,largely due to the reduction of metabolic stress,employment of proper hosts for improving pathway enzyme activities,and flexible adjustment of the relative biosynthetic strength between the coculture *** findings of this study extend the applicability of co-culture engineering in complex natural product biosynthesis.