Characteristics of mRNA dynamic expression related to spinal cord ischemia/reperfusion injury:a transcriptomics study

作     者：Zhi-ping Qi Peng Xia Ting-ting Hou Ding-yang Li Chang-jun Zheng Xiao-yu Yang 

作者机构：The Second HospitalJilin University 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2016年第11卷第3期

页      面：480-486页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China No.81350013 and 31572217 

主　　题：nerve regeneration spinal cord injury ischemia/reperfusion injury messenger RNA transcription oligonucleotide sequence microarray transcriptome c DNA sequence NADPH oxidase neural regeneration 

摘      要：Following spinal cord ischemia/reperfusion injury,an endogenous damage system is immediately activated and participates in a cascade *** is difficult to interpret dynamic changes in these pathways,but the examination of the transcriptome may provide some *** transcriptome reflects highly dynamic genomic and genetic information and can be seen as a precursor for the *** used DNA microarrays to measure the expression levels of dynamic evolution-related m RNA after spinal cord ischemia/reperfusion injury in *** abdominal aorta was blocked with a vascular clamp for 90 minutes and underwent reperfusion for 24 and 48 *** simple ischemia group and sham group served as *** rats had regained consciousness,hindlimbs showed varying degrees of functional impairment,and gradually improved with prolonged reperfusion in spinal cord ischemia/reperfusion injury ***-eosin staining demonstrated that neuronal injury and tissue edema were most severe in the 24-hour reperfusion group,and mitigated in the 48-hour reperfusion *** were 8,242 differentially expressed m RNAs obtained by Multi-Class Dif in the simple ischemia group,24-hour and 48-hour reperfusion *** m RNA dynamic expression patterns were obtained by Serial Test *** them,five patterns were *** the No.28 pattern,all differential genes were detected in the 24-hour reperfusion group,and their expressions showed a trend in ***.11 pattern showed a decreasing trend in m RNA whereas No.40 pattern showed an increasing trend in m RNA from ischemia to 48 hours of reperfusion,and peaked at 48 *** the No.25 and No.27 patterns,differential expression appeared only in the 24-hour and 48-hour reperfusion *** the five m RNA dynamic expression patterns,No.11 and No.40 patterns could distinguish normal spinal cord from pathological ***.25 and No.27 patterns could distinguish simple ischemia from ischemia/reperfu