Nano-hydroxyapatite formation via co-precipitation with chitosan-g-poly（N-isopropylacrylamide） in coil and globule states for tissue engineering application

作     者：Yang YU Hong ZHANG Hong SUN Dandan XING Fanglian YAO 

作者机构：School of Chemical Engineering and Technology Key Laboratory of Systems Bioengineering of Ministry of Education Tianjin University Tianjin 300072 China Consolidated Research Institute for Advanced Science and Medical Care Waseda University (ASMeW) Shinjuku-ku Tokyo 162-0041 Japan Basic Medical College Hebei United University Tangshan 063000 China 

出 版 物：《Frontiers of Chemical Science and Engineering》 (化学科学与工程前沿（英文版）)

年 卷 期：2013年第7卷第4期

页      面：388-400页

核心收录：

学科分类：080503[工学-材料加工工程] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070305[理学-高分子化学与物理] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 

基　　金：国家自然科学基金 国家科技部项目 

主　　题：chitosan poly（N-isopropylacrylamide）,hydroxyapatite coil globule bone tissue engineering 

摘      要：With the excellent biocompatibility and osteo- conductivity, nano-hydroxyapatite （nHA） has shown significant prospect in the biomedical applications. Con- trolling the size, crystallinity and surface properties of nHA crystals is a critical challenge in the design of HA based biomaterials. With the graft copolymer of chitosan and poly（N-isopropylacrylamide） in coil and globule states as a template respectively, a novel composite from chitosan-g-poly（N-isopropylacrylamide） and nano-hydro- xyapatite （CS-g-PNIPAM/nHA） was prepared via co- precipitation. Zeta potential analysis, thermogravimetric analysis and X-ray diffraction were used to identify the formation mechanism of the CS-g-PNIPAM/nHA compo- site and its morphology was observed by transmission electron microscopy. The results suggested that the physical aggregation states of the template polymer could induce or control the size, crystallinity and morphology of HA crystals in the CS-g-PNIPAM/nHA composite. The CS-g-PNIPAM/nHA composite was then introduced to chitosan-gelatin （CS-Gel） polyelectronic complex and the cytocompatibility of the resulting CS- Gel/composite hybrid film was evaluated. This hybrid film was proved to be favorable for the proliferation of MC 3T3-E1 cells. Therefore, the CS-g-PNIPAM/nHA compo- site is a potential biomaterial in bone tissue engineering.