Lentivirus carrying the Atoh1 gene infects normal rat cochlea

作     者：Song Pan Jingzhi Wan Shaosheng Liu Song Zhang Hao Xiong Jun Zhou Wu Xiong Kunfei Yu Yong Fu 

作者机构：Department of Otolaryngology Guangming New District People's Hospital Department of Surgery Hubei University of Science and Technology Department of Otolaryngology Beilun Branch of the First Affiliated Hospital College of Medicine Zhejiang University Department of Otolaryngology Second Affiliated Hospital Sun Yat-sen University Department of Otolaryngology First Affiliated Hospital College of Medicine Zhejiang University 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2013年第8卷第17期

页      面：1551-1559页

核心收录：

学科分类：1002[医学-临床医学] 100213[医学-耳鼻咽喉科学] 10[医学] 

基　　金：supported by grants from the National Basic Research Program of China (973 Program), No. 2012CB967900, 2012CB967904 the National Natural Science Foundation of China, No. 81070782 the Natural Science Foundation of Zhejiang Province, China, No. 30672308 the Qianjiang Talent Project of Science and Technology Ministry in Zhejiang Province, No. 2011R10014 the Natural Science Foundation of Ningbo, No. 2011A610042 

主　　题：neural regeneration gene therapy Atohl lentivirus hair cells myosin 7a cochlea auditorythreshold grants-supported paper neuroregeneration 

摘      要：Lentivirus carrying the Atohl gene can infect Corti＇s organ and express a hair-like cell surface marker in the supporting cell area. However, expression of the gene carried by adenovirus is instantaneous, which undoubtedly limits its clinical application. Lentivirus acts as a carrier that can stably and continuously express genes. In this study, the cochlear structure and hearing level were not affected, and Atohl gene carried by lentivirus promoted the production of hair-like cells in the cochlear supporting cell area. This led to expression of the hair-like cell surface marker myosin 7a 30 days after lentivirus carrying Atohl was microinjected into the cochlear round window of rats.