Polyhydroxybutyrate/Hydroxyapatite Highly Porous Scaffold for Small Bone Defects Replacement in the Nonload-bearing Parts

作     者：Fedor Senatov Natalia Anisimova Mikhail Kiselevskiy Aleksey Kopylov Viktor Tcherdyntsev Aleksey Maksimkin 

作者机构：National University of Science and Technology MISIS Leninskiy pr. 4 Moscow 119049 Russian Federation N.N. Blokhin Russian Cancer Research Center Kashirskoye sh. 23 Moscow 115478 Russian Federation 

出 版 物：《Journal of Bionic Engineering》 (仿生工程学报（英文版）)

年 卷 期：2017年第14卷第4期

页      面：648-658页

核心收录：

学科分类：080503[工学-材料加工工程] 081704[工学-应用化学] 07[理学] 08[工学] 070305[理学-高分子化学与物理] 0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0817[工学-化学工程与技术] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0836[工学-生物工程] 0702[理学-物理学] 

基　　金：supported by the Federal Targeted Program the financial support from the Ministry of Education and Science of Russian Federation support from the Ministry of Education and Science of the Russian Federation in the framework of increase Competitiveness Program ofNUST "MISIS” 

主　　题：polyhydroxybutyrate hydroxyapatite scaffold porous structure implant bone regeneration 

摘      要：In the present work, Polyhydroxybutyrate （PHB）/Hydroxyapatite （HA） porous composites （10%, 20%, 30 %, 40%, 50% weight HA） were obtained by sintering. PHB/20% HA optimally combines satisfactory mechanical properties with a high content of the bioactive component （HA）. Porous PHB/20% HA scaffolds have shown high mechanical properties （compressive strength of 106 MPa and Young＇s modulus of 901 MPa）. A high volume fraction of interconnected pores （〉 50 vol.%） was achieved with pore size of 50 grn - 500 gm. Biocompatibility of porous pure PHB and PHB/20%HA, as its osseointegration were assessed in vitro and after implantation in laboratory animals. PHB/20% HA （-5% ＋ 0.9%） and pure PHB ~3% ~ 1.4%） samples after 24 hours of incubation with human leucocytes showed no significant level of cytotoxicity when p = 0.648 （p-value）. In vitro massive adhesion of mouse Multipotent Mesenchymal Stromal Cells （MMSC） to the surface of both porous samples was shown. PHB/20% HA induced more intensive MMSC proliferation compared to pure PHB, which are 31% ＋ 6.1% and 20% ＋ 5.7 % respectively when p = 0.039. We observed the resorption （implant surface area was reduced by 49 %） and integration of the porous PHB/20% HA samples into surrounding tissues after 30 days of implantation. The signs of osteoclasts accumulation, neo-angigenesis and new bone formation were observed, which make PHB/20% HA promising for bone tissue engineering.