Preparation of Magnetic Chitosan Nanoparticles and Immobilization of Laccase
Preparation of Magnetic Chitosan Nanoparticles and Immobilization of Laccase作者机构：Key Laboratory of Fiber Optic Sensing Technology and Information Processing (Ministry of Education) Wuhan University of Technology
出 版 物：《Journal of Wuhan University of Technology(Materials Science)》 (武汉理工大学学报（材料科学英文版）)
年 卷 期：2009年第24卷第1期
页 面：42-47页
核心收录：
学科分类：07[理学] 070205[理学-凝聚态物理] 08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0702[理学-物理学]
基 金：Funded by Key Project of National Science Foundation of China (No.60537050) the National Science Foundation of China (No. 60377032)
主 题：laccase magnetic chitosan nanoparticles immobilization enzyme activity stability
摘 要：The magnetic chitosan nanoparticles were prepared by reversed-phase suspension method using Span-80 as an emulsifier, glutaraldehyde as cross-linking reagent. And the nanoparticles were characterized by TEM, FT-IR and hysteresis loop. The results show that the nanoparticles are spherical and almost superparamagnetic. The laccase was immobilized on nanoparticles by adsorption and subsequently by cross-linking with glutaraldehyde. The immobilization conditions and charac-terizations of the immobilized laccase were investigated. The optimal immobilization conditions were as follows： 10 mL of phosphate buffer （0.1 M, pH 7.0） containing 50 mg of magnetic chitosan nanoparticles, 1.0 mg·mL-1 of laccase and 1% （v/v） glutaraldehyde, immobilization temperature of 4 ℃ and immobilization time of 4 h. The immobilized laccase exhibited an appreciable catalytic capability （480 units·g-1 support） and had good storage stability and operation stability. The Km of immobilized and free laccase for ABTS were 140.6 and 31.1 μM in phosphate buffer （0.1 M, pH 3.0） at 37 ℃, respectively. The immobilized laccase is a good candidate for the research and development of biosensors based on laccase catalysis.
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