Research on the effects of CD137 signaling on the function of CD3^-CD56^+NK cells
Research on the effects of CD137 signaling on the function of CD3^-CD56^+NK cells作者机构:Cancer Biotherapy Center the First Affiliated Hospital of Nanjing Medical University Nanjing 210029 China
出 版 物:《Journal of Nanjing Medical University》 (南京医科大学学报(英文版))
年 卷 期:2009年第23卷第1期
页 面:10-14页
学科分类:0710[理学-生物学] 0810[工学-信息与通信工程] 0808[工学-电气工程] 1002[医学-临床医学] 100214[医学-肿瘤学] 0812[工学-计算机科学与技术(可授工学、理学学位)] 10[医学]
基 金:supported by National Nature Science Foundation of China (No.30772549)
主 题:CD137 CD3CD56+NK cells signal
摘 要:Objective: To investigate the effects of CD137 signaling on the regulation of CD3-CD56+NK cells function. Methods: CD3 CD56+NK cells were treated with CD137 mAb or mouse IgG 1 isotype control to study the effects of CD 137 signaling on the function of CD3-CD56+NK cells. Cytotoxicity was measured by LDH activity in the supernatants of cell cultures; NKG2D and LFA-I expression on CD3-CD56+NK cells were analyzed by flow cytometry. Results: CD137 was expressed on activated CD3-CD56+NK cells. The CD137 mAb enhanced the ability of CDB-CD56+NK cells to kill lung cancer cells(A549); Further studies revealed that the expression of NKG2D and LFA-1 was significantly increased in activated cells, and blockade of NKG2D and LFA-1 dramatically attenuated CD3CD56+NK cytolysis of A549 cancer cells. Conclusion: CD 137 signaling increases the ability of CD3-CD56+NK cells to kill cancer cells via up- regulating the expression of NKG2D and LFA-1.