Research on the effects of CD137 signaling on the function of CD3^-CD56^+NK cells

作     者：Yu Zhang Songwen Ju Yongqian Shu 

作者机构：Cancer Biotherapy Center the First Affiliated Hospital of Nanjing Medical University Nanjing 210029 China 

出 版 物：《Journal of Nanjing Medical University》 (南京医科大学学报（英文版）)

年 卷 期：2009年第23卷第1期

页      面：10-14页

学科分类：0710[理学-生物学] 0810[工学-信息与通信工程] 0808[工学-电气工程] 1002[医学-临床医学] 100214[医学-肿瘤学] 0812[工学-计算机科学与技术（可授工学、理学学位）] 10[医学] 

基　　金：supported by National Nature Science Foundation of China (No.30772549) 

主　　题：CD137 CD3CD56+NK cells signal 

摘      要：Objective： To investigate the effects of CD137 signaling on the regulation of CD3-CD56＋NK cells function. Methods： CD3 CD56＋NK cells were treated with CD137 mAb or mouse IgG 1 isotype control to study the effects of CD 137 signaling on the function of CD3-CD56＋NK cells. Cytotoxicity was measured by LDH activity in the supernatants of cell cultures; NKG2D and LFA-I expression on CD3-CD56＋NK cells were analyzed by flow cytometry. Results： CD137 was expressed on activated CD3-CD56＋NK cells. The CD137 mAb enhanced the ability of CDB-CD56＋NK cells to kill lung cancer cells（A549）; Further studies revealed that the expression of NKG2D and LFA-1 was significantly increased in activated cells, and blockade of NKG2D and LFA-1 dramatically attenuated CD3CD56＋NK cytolysis of A549 cancer cells. Conclusion： CD 137 signaling increases the ability of CD3-CD56＋NK cells to kill cancer cells via up- regulating the expression of NKG2D and LFA-1.