Effect of Stathmin Decoy-oligodeoxynucleotides on the Proliferation and Differentiation of Precartilainous Stem Cells

作     者：郭风劲 张衣北 陈安民 GUO Fengjing,ZHANG Yibei,CHEN Anmin Department of Orthopedics,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China

作者机构：Department of Orthopedics Tongji Hospital Tongji Medical College Huazhong University of Science and Technology 

出 版 物：《Journal of Huazhong University of Science and Technology(Medical Sciences)》 (华中科技大学学报（医学英德文版）)

年 卷 期：2007年第27卷第5期

页      面：557-560页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

基　　金：This project was supported by a grant from National Natural Sciences Foundation of China (No 30371439) 

主　　题：decoy-oligodeoxynucleotides Stathmin gene precartilainous stem cells proliferation differentiation 

摘      要：By using decoy-oligodeoxynucleotides （decoy-ODNS） technique, the effects of Stathmin gene on the proliferation and differentiation of in vitro cultured precartilainous stem cells （PSCs） were investigated. The Stathmin decoy-ODNs were transfected into PSCs in rats by using gene transfection technique. Under the induction of cortisol （1 μmol/L）, electrophoretic mobility shift assay was used the inhibitory effects of decoy-ODNS on Stathmin gene. MTT and cytometry were used to test the cell proliferation. The expression of collagen Ⅱ and Ⅴ and Stathmin protein was detected by using Western blot. The results showed that Stathmin decoy-ODNs inhibited the Stathmin activity in a dose-dependent manner. When the concentration of decoy-ODNs was 10 times of standard con- centration, the proliferation of PSCs was obviously suppressed and the differentiation happened. Compared to the control group, the difference was significant （P〈0.05）. It was concluded that decoy-ODNs could inhibit the proliferation and promote the differentiation of PSCs by antagonizing Stathmin activity.