Cloning and characterization of rice RH3 gene induced by brown planthopper
Cloning and characterization of rice RH3 gene induced by brown planthopper作者机构:Wuhan Univ Key Lab Minist Educ Plant Dev Biol Coll Life Sci Wuhan 430072 Peoples R China
出 版 物:《Chinese Science Bulletin》 (Chin. Sci. Bull.)
年 卷 期:2003年第48卷第18期
页 面:1976-1981页
核心收录:
学科分类:0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种]
基 金:National Natural Science Foundation of China NSFC (30170085)
主 题:brown planthopper histone H3 RNA in situ hybridiza-tion defense response.
摘 要:Experiments have showed that the histone H3 gene is correlated with development, cell speciality and stress response. The RH3 full-length cDNA was isolated from the cDNA library of rice infested by brown planthopper (BPH) with EST (Accession no. BU572343) screened from rice SSH library as probe. This gene encodes histone H3 protein in-cluding 136 amino acids, with one amino acid different from a kind of disease resistance-related protein in rice (AF467728). At the position 126, the aspartic acid is replaced by lysine. The time course results showed that the expression of the RH3 began to increase at 8 h after BPH-feeding, and got to its peak at 96 h. Regulations of the gene expression in treatments with stress/defense signal molecules were ana-lyzed by Northern blot. Water deficit and Pyricularia grisea increased the expression of RH3 while ABA down-regulated the gene. The enhanced accumulation of RH3 transcripts in the vascular bundle and short cell of stem after BPH feeding was revealed by RNA in situ hybridization. It is the first time to report that RH3 is correlated with the response of rice to BPH.