Optimizing a nitrogen-supplemented, condensed corn soluble medium for growth of the Polyhydroxyalkanoate producer Pseudomonas putida KT217

作     者：Jeremy Javers William Gibbons Chinnadurai Karunanithy 

作者机构：ICMInc.ColwichKansasKSUSA Biology and Microbiology DepartmentSouth Dakota State UniversityBrookings SD57007 USA Food EngineeringFood and NutritionUniversity of Wisconsin-StoutMenomonie WI 54751 USA 

出 版 物：《International Journal of Agricultural and Biological Engineering》 (国际农业与生物工程学报（英文）)

年 卷 期：2012年第5卷第4期

页      面：62-67页

核心收录：

学科分类：0202[经济学-应用经济学] 02[经济学] 020205[经济学-产业经济学] 

基　　金：funding provided by the South Dakota Corn Utilization Council 

主　　题：cell density cell dry weight(CDW) biodegradable plastic biocreator pH nitrogen supplement growth rate 

摘      要：Pseudomonas putida KT217 produces medium-chain-length polyhydroxyalkanoate(mcl-PHA)that is of commercial interest as a biodegradable *** reduce PHA production costs,a less expensive medium to grow *** KT217 to a high cell dry weight(CDW)was *** KT217 was grown in aerated shake flasks on a condensed corn solubles(CCS)based medium that provided several organic acids and carbohydrates that were utilized for *** medium was prepared by adding various amounts of fresh CCS(100-600 g/L,or 34.9-209.4 g/L dry basis)to water and then centrifuging and *** CCS permeate media contained dry matter levels of 28.8-164.9 g/*** CCS permeate medium containing 108.4 g/L solids produced approximately 6 g CDW/L,at a growth rate of 1.03 per hour,and maximum cell population of 4×109 CFU/*** CCS is low in nitrogen,ammonium hydroxide was added at a level of 1.73 g/L,and this significantly increased the CDW(20 g/L)produced in an aerated ***,a nitrogen supplemented CCS medium could provide a cheap source of nutrients for production of *** KT217 and PHA.