Effect of collagen and collagen peptides from bluefin tuna abdominal skin on cancer cells
Effect of collagen and collagen peptides from bluefin tuna abdominal skin on cancer cells作者机构：Department of Applied Biological Chemistry Graduate School of Agriculture Kinki University
出 版 物：《Health》 (健康（英文）)
年 卷 期：2011年第3卷第3期
页 面：129-134页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Bluefin Tuna Collagen Type I Collagen Hepg2 Cell Hela Cell
摘 要：In the present study, we investigated the effect of collagen and collagen peptides from bluefin tuna abdominal skin on cancer cells. Collagens were extracted from bluefin tuna (Thunnus orientails) abdominal, mackerel, and carp skin. The calf and salmon collagen were used reagent grade as a standard samples. The main protein band pattern produced by SDS-PAGE of all collagen samples consisted of two chains and one chain. For collagen peptides samples, bluefin tuna ab-dominal skin collagen and salmon skin collagen were hydrolyzed by trypsin. Among samples, salmon, mackerel, carp collagen, and their collagen peptides did not significantly reduce relative cell growth. However, the bluefin tuna abdominal skin collagen dramatically reduced HepG2 and HeLa cell growth by over 50% relative in a concentration-dependent manner when added to cells seeded 96-well plates. This suggests the collagen adding time was mightily important for effect of the collagen.
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