Changes in expression of inhibitory substances in the intramural neurons of the stomach following streptozotocininduced diabetes in the pig

作     者：Michal Bulc Katarzyna Palus Lukasz Zielonka Magdalena Gajecka Jaroslaw Całka 

作者机构：Department of Clinical Physiology Faculty of Veterinary MedicineUniversity of Warmia and Mazury Department of Veterinary Prevention and Feed HygieneFaculty of Veterinary MedicineUniversity of Warmia and Mazury in Olsztyn 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2017年第23卷第33期

页      面：6088-6099页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by a statutory Grant No.528-0523.0802 KNOW(Leading National Research Centre)Scientific Consortium“Healthy Animal-Safe Food”,decision of Ministry of Science and Higher Education,No.05-1/KNOW2/2015 

主　　题：Immunohistochemistry Inhibitory neurons Streptozotocin Hyperglycemia Pig 

摘      要：AIM Influence of chronic hyperglycemia on chemical coding of enteric neurons in stomach using pig as a model for human diabetic *** Ten pigs were divided into two groups: diabetic(D group,n = 5) and control(C group,n = 5). Pigs constituting the experimental group were given streptozotocin(150 mg/kg). Animals were euthanized six weeks after the induction of diabetes. The samples of stomach were collected from animals of both groups. The cryostat sections were processed for double immunofluorescence staining using primary antisera directed towards pan-neuronal marker(Hu C/D) proteins and/or neuronal isoform of nitric oxide synthase(n NOS),vasoactive intestinal peptide(VIP) and galanin(GAL). RESULTS In the control group in the myenteric ganglia(MG) of the corpus we have noted 22.28% ± 1.19% of n NOS positive neurons,while in diabetic group we have found 40.74% ± 2.22% of n NOS immunoreactive perikarya(increase by 82.85 %). In turn in the pylorus we have observed 15.91% ± 0.58% n NOS containing neurons in control animals and 35.38% ± 1.54% in the diabetes group(increase by 122.37%). In the MG of the antrum and submucosal ganglion(SG) in the corpus hyperglycemia did not cause statistically significant changes. With regard to VIP-positive cell bodies in the antrum MG in the control animals we have noted 18.38 ± 1.39% and 40.74% ± 1.77% in the experimental group(increase by 121.65%). While in the corpus we have observed 23.20% ± 0.23% in the control and 30.93% ± 0.86% in the diabetes group(increase by 33.31%). In turn in the pylorus VIP positive cells bodies constituted 23.64% ± 1.56% in the control group and 31.20% ± 1.10% in the experimental group(increase by 31.97%). In the submucosal ganglion in the corpus we have noted 43.61% ± 1.06% in the control animals and 37.00% ± 1.77% in the experimental group(decrease by 15.15%). Expression of GAL-positive perikarya showed statistically significant changes only in the MG of the antrum and pylorus. In the antr