CHOP THER APY INDUCED MITOCHONDRIAL REDOX STATE ALTERATION IN NON-HODGKIN'S LYMPHOMA XENOGRAFTS

作     者：H.N.XU H.ZHAO T.A.MIR S.C.LEE M.FENG R.CHOE J.D.GLICKSON L.Z.LI 

作者机构：Department of Radiology University of PennsylvaniaPhiladelphiaPAUSA Britton Chance Laboratory of Redox Imaging Johnson Research Foundation Department of Biochemistry and Biophysics University of PennsylvaniaPhiladelphiaPAUSA Biostatistics and Data Management Core The Children's Hospital of Philadelphia(CHOP)PhiladelphiaPAUSA Department of Biomedical Engineering University of RochesterRochesterNYUSA Abramson Cancer Center University of PennsylvaniaPhiladelphiaPAUSA Institute of Translational Medicine and Therapeutics University of PennsylvaniaPhiladelphiaPAUSA 

出 版 物：《Journal of Innovative Optical Health Sciences》 (创新光学健康科学杂志（英文）)

年 卷 期：2013年第6卷第2期

页      面：29-35页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：The grant support to this work incude the Center of Magnetic Resonance and Optical Imaging(CMROI)-an NIH-supported research resource P41RR02305(R.Reddy) the Small A nimal Imaging Resources Program(SAIR)2U24-CA083105(J.D.Glickson and L.Chodosh) 2R01-CA101700(J.D.Glickson) NIH K99/R00-CA 126187(R.Choe) 

主　　题：NA DH flavoprotein DLCL2 therapeutic effect tumor metabolism 

摘      要：We are interested in investigating whether cancer therapy may alter the mitochondrial redox state in cancer cells to inhibit their growth and *** redox state can be imaged by the redox scanner that collects the fuorescence signals from both the oxidized-fAavoproteins(Fp)and the reduced form of nicotinamide adenine dinucleotide(NADH)in snap frozen tissues and has been previously employed to study tumor aggressiveness and treatment ***,with the redox scanner we investigated the effects of chemotherapy on mouse xenografts of a human diffuse large *** lymphoma cell line(DLCL2).The mice were treated with CHOP therapy,i.e,cyclophosphamide(C)+hydroxydoxorubicin(H)+Oncovin(O)+prednisone(P)with CHO administration on day 1 and prednisone administration on days *** Fp content of the treated group was significantly decreased(p=0.033)on day 5,and the mitochondrial redox state of the treated group was slightly more reduced than that of the control group(p=0.048).The decrease of the Fp heterogeneity(measured by the mean st andard deviation)had a border-line statistical significance(p=0.071).The result suggests that the mitochondrial metabolism of lymphoma cells was slightly suppressed and the lymphomas became less aggressive after the CHOP therapy.