Transforming growth factor-α promotes mouse blastocyst outgrowth and secretion of matrix metalloproteinases

作     者：陈舒怡 曹宇静 曾国庆 段恩奎 CHEN Shuyi;CAO Yujing;ZENG Guoqing;DUAN Enkui

作者机构：中国科学院动物研究所计划生育生殖生物学国家重点实验室北京100080 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2001年第114卷第12期

页      面：68-72,108页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071008[理学-发育生物学] 

基　　金：SpecialFundsforMajorStateBasicResearchProject (No G19990 5 5 90 3) theClimbingProjectofChina(No 92 0 2 110 193)andthe‘100scientists’program 

主　　题：transforming growth factor α · embryo implantation · attachment · outgrowth · matrix metalloproteinases 

摘      要：Objective To study the effect of transforming growth factor-α (TGF-α) on early stage of embryo *** Mouse blastocysts were cultured in vitro in medium containing various concentrations of TGF-α. Blastocyst implantation capacity was evaluated by calculating the percentage of embryos with attachment or outgrowth. Matrix metalloproteinases (MMPs) secretion of blastocysts was observed using gelatin zymography. Results There was no significant difference in the percentage of attachment between control and TGF-α treated groups, but the percentage of outgrowth of TGF-α treated groups was significantly higher than that of the control group after 24h culturing. Gelatin zymography showed that blastocysts cultured in TGF-α treated groups started secreting MMPs earlier than those in the control *** TGF-α is involved in regulating the mouse embryo implantation process by promoting blastocyst outgrowth and secreting matrix matalloproteinases.