Visible fluorescence of biological fluids as a renal failure marker:New integrative approach

作     者：Artur I.Kuznetsov Aleksander Frorip Jekaterina Kozlova Vitali Nagirnyi Mai Ots-Rosenberg Ivo Romet Alar Sünter 

作者机构：Tartu Science ParkAS Ldiamon Riia str.18551014 TartuEstonia Institute of PhysicsTartu University Ravila str.14^(c)Tartu 50411Estonia Department of Internal MedicineTartu University Puusepa str.8Tartu 51014Estonia BiomedicumTartu University Ravila str.1950411 TartuEstonia 

出 版 物：《Journal of Innovative Optical Health Sciences》 (创新光学健康科学杂志（英文）)

年 卷 期：2015年第8卷第4期

页      面：69-84页

核心收录：

学科分类：07[理学] 0701[理学-数学] 070101[理学-基础数学] 

基　　金：support for this work by the Archimedes project 3.2.1101.12-0011 supported in part by the institutional research funding IUT (IUT 2-26)of the Estonian Ministry of Education and Research 

主　　题：Auto-fuorescence of biological fuids AGE glycosides end stage renal diseases MALDI of human metabolites fluorescence of graphene oxide 

摘      要：Solid phases of visible fuorescence substance(VFS)of biological fuids(blood,urine,hemodia-lysate)which was proposed earlier as a morbidity and mortality marker by renal failure and diabetes were investigated in-depth by the methods of electron and confocal microscopy,optical spectroscopy and matrix assisted laser desorption-ionization(MALDI)mass *** is shown that dry VFS exists predominantly in the form of carbon-oxygen-nitrogen(N≈8.7 wt.%)nanoparticles(NPs)(5≤d≤100nm).For the first time the existence of the threshold energy E_(g)≈2.15eV for excitation of VFS was observed experimentally and confirmed by semi empirical calculations of the bathochromic shift.A good accordance with the earlier autonomous theo-retical calculations was ***,the long wavelength limit(575 nm)of the spectral range where the VFS can be used as a fluorescent marker was reliably determined.A pilot MALDI comparative study of graphene oxide(GO)and urine VFS was carried *** kinds of nitrogen-free particles(412≤M≤456 Da)were observed in each substance and possible computer models of those have been *** is established that along with nitrogen-containing advanced glycation end products(AGEs)also nitrogen-fee carbon-oxygen-lhydrogen particles(probably toxic)with the composition and structure related to GO can exist in *** types of particles should be taken into account in search for the reasons of high mortality among end stage renal disease patients.