The genetic variation of Angiostrongylus cantonensis in the People’s Republic of China

作     者：Shan Lv Yi Zhang Peter Steinmann Jurg Utzinger Xiao-Nong Zhou Lv Shan;Zhang Yi;Steinmann Peter;Utzinger Jurg;Zhou Xiao-Nong

作者机构：National Institute of Parasitic DiseasesChinese Center for Disease Control and PreventionShanghai 200025People’s Republic of China Swiss Tropical and Public Health InstituteP.O.BoxCH-4002 BaselSwitzerland University of BaselP.O.BoxCH-4003 BaselSwitzerland 

出 版 物：《Infectious Diseases of Poverty》 (贫困所致传染病（英文）)

年 卷 期：2017年第6卷第1期

页      面：1100-1110页

核心收录：

学科分类：0401[教育学-教育学] 04[教育学] 

基　　金：This work was supported by International Development and Research Centre(IDRC Canada)(grant no.105509-00001002-023) the International Society of Infectious Diseases(small grant 2007 fall) 

主　　题：Angiostrongylus cantonensis Genetics cox1 nad1 Internal transcribed spacer People’s Republic of China 

摘      要：Background:The People’s Republic of China(***)is the presumptive home range of the rat lungworm Angiostrongylus cantonensis,a major aetiological agent of human eosinophilic *** present a study of the genetic variation of *** in *** aim was to deepen the current knowledge pertaining to its origin and global spread from a molecular ***:Adult *** were collected in the frame of a national survey and identified based on morphological *** chain reaction(PCR)was employed to amplify the target DNA sequences(cytochrome c oxidase subunit I(cox1),nicotinamide adenine dinucleotide dehydrogenase subunit 1(nad1)and internal transcribed spacer(ITS)).The PCR product of cox1 was directly submitted to sequencing,while clone sequencing was used for nad1 and *** identity of the samples was verified by comparing the sequences to those of accepted *** *** specific composition of substitutions in each gene was analysed,and the genotypes were compared based on the complete cox1,nad1 and ITS ***:We characterised the complete mitochondrial genes cox1 and nad1 of 130 specimens and obtained 357 nuclear sequences containing two complete ITS(ITS1 and ITS2)and 5.8S rRNA of the same *** specimens were genetically confirmed as *** major groups(i.e.I and II)were identified according to the phylogeny of cox1 *** I could be further categorised into six distinct *** half of the specimens(47.7%)belong to the clade Ia and 22.3%to the group *** former was widely distributed across the study region.A variable number of repeat units in three microsatellites was observed,resulting in considerable length variation in *** variation of ITS sequences was found in a large proportion of the *** showed a striking difference between mitochondrial DNA and ***:Our results demonstrate that *** is the only rat lung