Thansgenic peanut plants obtained by particle bombardment via somatic embryogenesis regeneration system
Thansgenic peanut plants obtained by particle bombardment via somatic embryogenesis regeneration system作者机构：中科院上海植物生理生态所 上海 200032
出 版 物：《Cell Research》 (细胞研究(英文版))
年 卷 期：2001年第2期
页 面：156-160页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学]
基 金：the Natinnal Biotechnology Reseaxch Project of 863 High Technology contract No. 101-01-01-02
主 题：Peanut (Arachis hypogaea) somatic embryo hygromycin resistance particle bombardment.
摘 要：After pre-culture and treatment of osmosis, cotyledons of immature peanut (Arachis hypogaea L.) zygotic embryos were transformed via particle bombardment with a plasmid containing a chimeric hph gene conferring resistance to hygromycin and a chimeric intron-gus gene. Selection for hygromycin resistant calluses and somatic embryos was initiated at 10th d post-bombardment on medium containing 10-25 mg/L hygromycin. Under continuous selection, hygromycin resistant plantlets were regenerated from somatic embryos and were recovered from nearly 1.6% of the bombarded cotyledons. The presence and integration of foreign DNA in regenerated hygromycin resistant plants was confirmed by PCR (polymerase chain reaction) for the intron-gus gene and by Southern hybridization of the hph gene. GUS enzyme activity was detected in leaflets from transgenic plants but not from control, non-transformed plants. The production of transgenic plants are mainly based on a newly improved somatic embryogenesis regeneration system developed by us.
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